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EC number: 629-850-6 | CAS number: 1245638-61-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996 to 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to standard guidelines in compliance with GLP. Pentaerythritol triacrylate is a constituent of PETIA.
Cross-reference
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 005
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Method: MacGregor JT et al (1990)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 2-Propenoic acid, reaction products with pentaerythritol
- EC Number:
- 629-850-6
- Cas Number:
- 1245638-61-2
- Molecular formula:
- Not available for this UVCB.
- IUPAC Name:
- 2-Propenoic acid, reaction products with pentaerythritol
- Test material form:
- other: Colorless or light amber nonvolatile liquid
- Details on test material:
- - Name of test material: Pentaerythritol triacrylate
- Molecular formula (if other than submission substance): C14H18O7
- Molecular weight (if other than submission substance): 298.3
- Physical state: Liquid (also occurs as a semisolid or crystalline solid at temperatures up to 40°C)
- Analytical purity: 45% (for lot HCC0340)
- Impurities: HPLC indicated a major peak, seven impurity components with areas greater than 1% of the major peak area, and nine impurity components with relative areas between 0.5% and 1%. The impurities were tentatively identified as structurally related adducts, dimers, and acrylates as well as trimethylolpropane triacrylate and its related esters and adducts.
- Lot/batch No.: HCC0340
- Stability under test conditions: No degradation of the bulk chemical was detected
- Storage condition of test material: Stored at room temperature, protected from light in amber glass bottles with Teflon®-lined lids.
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS:
- Source: Taconic Laboratory Animals and Service (Germantown, NY)
- Age at study initiation: 6 weeks
- Assigned to test groups randomly: Yes
- Housing: Individually housed in polycarbonate cages
- Diet (e.g. ad libitum): NTP-2000 pelleted diet (Zeigler Brothers, Inc., Gardners, PA), ad libitum, changed weekly
- Water (e.g. ad libitum): Tap water (Columbus municipal supply), ad libitum
- Acclimation period: 11-15 d
ENVIRONMENTAL CONDITIONS
- Temperature (°F): 72 ± 3 °F
- Humidity (%): 50 ± 15 %
- Air changes (per h): 10/h
- Photoperiod (h dark / h light): 12 h dark / 12 h fluorescent light
Administration / exposure
- Route of administration:
- dermal
- Vehicle:
- - Vehicle(s)/solvent(s) used: Acetone
- Details on exposure:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2 mL/kg bw - Duration of treatment / exposure:
- 3 months
- Frequency of treatment:
- 5 days per week for 14 weeks
- Post exposure period:
- No data
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0.75, 1.5, 3, 6 and 12 mg/kg bw
Basis:
nominal conc.
- No. of animals per sex per dose:
- 10
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- No data
Examinations
- Tissues and cell types examined:
- Peripheral blood samples
- Details of tissue and slide preparation:
- TREATMENT AND SAMPLING TIMES: Peripheral blood samples were obtained from male and female mice at the end of the study
DETAILS OF SLIDE PREPARATION: Smears were immediately prepared and fixed in absolute methanol. The methanol fixed slides were stained with acridine orange and coded.
METHOD OF ANALYSIS: Slides were scanned to determine the frequency of micronuclei in 2,000 normochromatic erythrocytes (NCEs) per animal. In addition, the normochromatic/polychromatic erythrocyte (NCE/PCE) ratio in 1,000 total erythrocytes per animal was determined to provide a measure of chemical-induced bone marrow toxicity. - Evaluation criteria:
- No data
- Statistics:
- - Results were analyzed by a statistical software package
- Significance of micronucleated NCEs/1,000 NCEs tested by the one-tailed Cochran-Armitage trend test (significant at P ≤ 0.025), followed by Pairwise comparison with the vehicle controls (significant at P ≤ 0.005)
Results and discussion
Test results
- Key result
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Additional information on results:
- RESULTS OF DEFINITIVE STUDY
- Induction of micronuclei (for Micronucleus assay): See Table 1
- Ratio of NCE/PCE (for Micronucleus assay): See Table 1
Any other information on results incl. tables
Table 1: Frequency of micronuclei in peripheral blood normochromatic erythrocytes of B6C3F1 mice following dermal application of pentaerythritol triacrylate for 3 monthsa
Compound |
Dose (mg/kg) |
Number of Mice with Erythrocytes Scored |
Micronucleated NCEs/ 1,000 NCEsb |
P Valuec |
NCEsb(%) |
Male |
|||||
Acetoned |
|
10 |
1.20 ± 0.21 |
|
98.2 ± 0.1 |
Pentaerythritol triacrylate
|
0.75 |
10 |
0.75 ± 0.15 |
0.9253 |
98.1 ± 0.1 |
1.5 |
10 |
1.00 ± 0.15 |
0.7269 |
98.4 ± 0.1 |
|
3 |
10 |
0.90 ± 0.19 |
0.8229 |
98.3 ± 0.1 |
|
6 |
10 |
1.05 ± 0.22 |
0.6727 |
98.4 ± 0.1 |
|
12 |
10 |
1.05 ± 0.16 |
0.6727 |
98.2 ± 0.1 |
|
|
|
P=0.394e |
|
|
|
Female |
|||||
Acetone |
|
9 |
0.72 ± 0.15 |
|
98.2 ± 0.1 |
Pentaerythritol triacrylate
|
0.75 |
10 |
0.65 ± 0.15 |
0.606 |
98.1 ± 0.1 |
1.5 |
9 |
0.78 ± 0.19 |
0.4237 |
98.3 ± 0.1 |
|
3 |
10 |
0.65 ± 0.17 |
0.606 |
98.3 ± 0.1 |
|
6 |
10 |
0.90 ± 0.18 |
0.2722 |
98.3 ± 0.1 |
|
12 |
10 |
0.85 ± 0.11 |
0.329 |
98.4 ± 0.1 |
|
|
|
P=0.210 |
|
|
aStudy was performed at SITEK Research Laboratories, Inc.
NCE=normochromatic erythrocyte
bMean ± standard error
cPairwise comparison with the vehicle controls, significant at P ≤ 0.005
dVehicle control
eSignificance of micronucleated NCEs/1,000 NCEs tested by the one-tailed Cochran-Armitage trend test, significant at P ≤ 0.025
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Under the conditions of this mouse micronucleus assay, the test substance was negative for mutagenic activity. - Executive summary:
A study was conducted to determine the mutagenicity potential of PETIA in the mouse peripheral blood micronucleus test.
The substance (0.75 - 12 mg/kg bw) was administered dermally to male and female B6C3F1 mice for 3 months. Peripheral blood samples were obtained at the end of the study and smears slides were immediately prepared and scanned to determine the frequency of micronuclei in 2,000 normochromatic erythrocytes (NCEs) per animal. In addition, the normochromatic erythrocytes/polychromatic erythrocyte (NCE/PCE) ratio in 1,000 total erythrocytes per animal was determined to provide a measure of chemical-induced bone marrow toxicity.
No increase in the frequency of micronucleated NCEs and NCE/PCE ratios were observed in peripheral blood samples from the male or female B6C3F1 mice.
Under the conditions of the study, the test substance was considered to be negative for mutagenic activity.
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