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EC number: 283-829-2
CAS number: 84731-70-4
No contaminant colonies were observed on the sterility plates for the
highest concentration of the test substance or the S9 mix.
Confirmatory mutagenicity study:
There was no increase in the number of revertant colonies compared to
the vehicle control at any concentration of the test substance either in
the presence or absence of metabolic activation system in any strain
tested. The precipitation of test item was observed at the concentration
more than 250µg/plate
in all strains used, when test item solutions were mixed with top agar.
Precipitation was observed at the 500µg/plate
concentration after 48 h incubation. The number of viable cells in the
overnight cultures as determined by optical density was within
acceptable limits: 1.2 to 2.3E09 cells/mL (S. typhimurium strains) and
2.3E09 cells/mL (E. coli strain); 1.1 to 2.2E09 cells/mL (S. typhimurium
strains) and 2.1E09 cells/mL (E. coli strain) in the 1st and 2nd
experiments respectively. The positive controls induced a significant
increase in the number of revertant colonies indicating the assay was
Dosing solutions were analysed. The concentration analyses indicate that
the actual concentration of the dosing solutions used were 101.81 % to
103.82 % and 94.47 % to 103.81 % of target concentration in the 1st and
2nd experiments, respectively.
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