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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Remarks:
Growth inhibition of Pseudomonas putida
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
Not reported
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
KL2 due to read across
Justification for type of information:
Refer to the section 13 for details on the category justification.
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
yes
Remarks:
Highest concentration tested was 10,00 g/L upon request of Study Sponsor; due to nature of the material, test temperature was 25 +/- 1°C instead of 21 +/- 1°C, cultures plated on full agar plates instead of Erlenmeyers and duration 72 h instead of 16 h
Principles of method if other than guideline:
Not applicable
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Maximal soluble amount of test material (saturation concentration) diluted in doubly distilled water. Test concentrations prepared by serial dilution of initial saturated solution.
Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida MIGULA, strain Berlin 33/2, from the German Microorganism culture Collection (D-3300 Braunschweig)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Post exposure observation period:
None
Test temperature:
25 +/- 1°C
Nominal and measured concentrations:
- Nominal: 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L
Details on test conditions:
- Temperature: 25 +/- 1°C
Reference substance (positive control):
no
Key result
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
ca. 0.83 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Key result
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
ca. 6 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

None.

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the study, the nominal 16 h EC50 and EC10 were 6.0 and 0.83 g/L (i.e. 6000 and 830 mg/L), respectively.
Executive summary:

A study was conducted to assess the toxicity of the read across substance, C8-18 and C18-unsatd. DEA, to the bacteria Pseudomonas putida according to DIN Guideline 38412 L8, in compliance with GLP. Pseudomonas putida colonies, in triplicate, were exposed to the test substance (directly diluted in test medium) at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 16 h. Under the conditions of the study, the nominal 16 h EC50 and EC10 were 6.0 and 0.83 g/L (i.e. 6000 and 830 mg/L), respectively (Poth, 1992).

Description of key information

Key value for chemical safety assessment

EC50 for microorganisms:
6 000 mg/L
EC10 or NOEC for microorganisms:
830 mg/L

Additional information

A study was conducted to assess the toxicity of the read across substance, C8-18 and C18-unsatd. DEA, to the bacteria Pseudomonas putida according to DIN Guideline 38412 L8, in compliance with GLP. Pseudomonas putida colonies, in triplicate, were exposed to the test substance (directly diluted in test medium) at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 16 h. Under the conditions of the study, the nominal 16 h EC50 and EC10 were 6.0 and 0.83 g/L (i.e. 6000 and 830 mg/L), respectively (Poth, 1992).