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EC number: 931-335-9
CAS number: 90622-74-5
results: (refer to the table in the attached word document under
'attached background material')
analytically determined concentrations of the test substance in the
analysed test media varied in the range from 61% to 112% of the nominal
values. The mean measured values, calculated over all measurements
during the test period amounted to 79% of nominal at the concentration
of nominal 0.10 mg/L, to 81% at the concentration of nominal 0.32 mg/L,
and to 83 % at the concentration of nominal 1.0 mg/L. Therefore, the
biological results were based on the nominal concentrations as well on
the mean measured test substance concentrations.
the stock solution samples, the measured concentrations amounted to in
the mean 95% of the nominal values. The test substance was sufficiently
stable in the stock solutions under the test conditions during the
longest stock solution renewal periods of 4 days.
on observed results:
The death of one fish at the test concentrations of nominal 0.10 and
0.32 mg test substance/L on Day 4 was not determined as a significant
toxic effect of the test substance, since no clear symptoms of
intoxication were observed at the test fish at these test
concentrations, and according to the test guideline the death of one
fish is tolerated even in the control.
The reduced food uptake observed from Day 7 to 12 at the test
concentration of nominal 0.32 mg/L (some uneaten food was observed at
the bottom of the aquarium) had no significant effect on the growth rate
of the test fish and was therefore not assessed as a significant toxic
A study was carried out to determine
the chronic toxicity of the test substance, C12-18 and C18-unsatd. DEA,
to Rainbow trout (Oncorhynchus mykiss) according to OECD Guidelines 204
and 215, in compliance with GLP. Ten fish per group were exposed to the
test substance (dissolved in water using intense stirring) at 0.032,
0.10, 0.32, 1.0 and 3.2 mg/L for 28 d under flowthrough conditions.
Mortality and symptoms of toxicity were observed daily and the growth
parameters (i.e. body length and weight) were recorded on Days 0 and 28.
For analytical dose verification (HPLC-UV), samples were taken in the
control, 0.10 and 0.32 mg/L groups on Days 0, 7, 14, 20 and 28 of
exposure. At 3.2 mg/L, all fish were dead after one day. At 1.0 mg/L,
all fish showed clear symptoms of toxicity and died by Day 25. No
significant effect on mortality or signs of intoxication were observed
up to the concentration of 0.32 mg/L. According to the results of a
Williams test, the mean body length and mean body wet weight of the
surviving fish were not significantly reduced compared to controls at up
to and including the dose of 0.32 mg/L. Growth rates at higher
concentrations could not be determined due to mortality. Under the study
conditions, the nominal 28 d NOEC and LOEC for mortality, symptoms of
toxicity and growth were established at 0.32 and 1.0 mg/L (equivalent to
measured concentrations of 0.26 and 0.83 mg/L), respectively (Memmert,
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