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Ecotoxicological information

Long-term toxicity to fish

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Description of key information

The NOEC and LOEC for rainbow trout exposed to amides, C12-18 (even numbered) and C18-unsatd., N,N-bis(hydroxyethyl) for 28 days were established at nominal concentrations of 0.32 and 1.0 mg/L (i.e., equivalent to measured concentrations of 0.26 and 0.83 mg/L), respectively. The lowest lethal concentration was 1.0 mg/L (i.e., equivalent to a measured concentration of 0.83 mg/L).

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
0.32 mg/L

Additional information

A study was carried out to determine the effects in rainbow trout of exposure to amides, C12-18 (even numbered) and C18-unsatd., N, N-bis(hydroxyethyl) for 28 days. The study was conducted according to OECD guidelines 204 and 215. Ten fish per glass vessel were exposed to the test substance at 0.032, 0.10, 0.32, 1.0 and 3.2 mg/L under flow through conditions. Mortality and symptoms of toxicity were observed daily and the growth parameters (i.e., body length and weight) were recorded on Days 0 and 28. At the highest nominal test concentration of 3.2 mg/L, all fish were dead after one day. At nominal concentration of 1.0 mg/L, all fish showed clear symptoms of toxicity and died by Day 25. No significant effect on mortality or signs of intoxications was observed up to a nominal test concentration of 0.32 mg/L. Further, according to the results of Williams-Tests, the mean body length and mean body wet weight of the surviving fish were not significantly reduced compared to the control up to and including 0.32 mg/L. Growth rates at higher concentrations could not be determined due to mortality. Under the test conditions, the NOEC and LOEC of amides, C12-18 (even numbered) and C18-unsatd., N, N-bis(hydroxyethyl) for mortality, symptoms of toxicity and effects on growth was established at nominal concentrations of 0.32 and1.0 mg/L (i.e., equivalent to a measured concentration of 0.26 and 0.83 mg/L), respectively, in rainbow trout