4-[(morpholinothio)thioxomethyl]morpholine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Regulations for the Enforcement of the Federal Hazardous Substances act (September 17 1964)

Principles of method if other than guideline:

To measure the toxic effect after 1 hour inhalation to 10 male albino rats.

GLP compliance:

not specified

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

4-[(morpholinothio)thioxomethyl]morpholine

EC Number:

237-335-9

EC Name:

4-[(morpholinothio)thioxomethyl]morpholine

Cas Number:

13752-51-7

Molecular formula:

C9H16N2O2S2

IUPAC Name:

morpholin-4-yl morpholine-4-carbodithioate

Constituent 2

Reference substance name:

Good-Rite® 3030x18 (Cure-Rite® 18); purity: not noted

IUPAC Name:

Good-Rite® 3030x18 (Cure-Rite® 18); purity: not noted

Test animals

Species:

rat

Strain:

other: albino

Sex:

male

Administration / exposure

Route of administration:

inhalation: dust

Type of inhalation exposure:

not specified

Vehicle:

air

Details on inhalation exposure:

rats were exposed to the test material in an inhalation chamber for one hour, at a determined concentration of 164.4mg/L.
Chamber - plexiglass jar fitted with plexiglass lid. Lid contained air intake and exit tubes positioned at the top and bottom of the chamber
respectively. Sample was introduced into the chamber atmosphere by leading the intake air to the bottom of a filter flask containing the sample. The
air stream was directed tangentially against the walls of the flask to produce a swirling motion. The air flow with entrained sample was then directed into the chamber atmosphere.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

1 h

Concentrations:

164.4 mg/L

No. of animals per sex per dose:

10 males in one group.

Control animals:

no

Details on study design:

Exposure was 1 hour to the rats in the chamber. Rats were then housed in wire mesh cages suspended above the droppings. Observations were made continuously of the appearance and behaviour of the animals during the exposure period and at frequent intervals thereafter for the total 14 days.
at end of observation period, rats were weighed, sacrificed by cerebral concussion and gross necropsies were performed.

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC0

Effect level:

164.4 mg/L air (nominal)

Based on:

test mat.

Remarks:

Cure-rite 18

Exp. duration:

1 h

Remarks on result:

other: no confidence limits were calculated during this study

Mortality:

No mortalities occurred in the group of 10 male rats.

Clinical signs:

other: serosanguineous lacrimation and nasal discharge was noted in occasional rats in first few days of the study. Followed by normal appearance for the remainder of the 14 day observation period.

Body weight:

Average body weight gain of 52 grams which is within normal limits for rats of the age, sex and strain used.

Gross pathology:

no pathological alterations.

Other findings:

observations included "excited" activity upon initiation and intermittent preening, excessive masticatory movements, slight excessive lacrimation,
rapid respiration and hair coats covered with sample dust throughout the hour. Cloudiness of the chamber atmosphere prevented more detailed
observations.

Removal from the chamber - observations showed sample covered hair coats and excessive lacrimation in all rats, and excessive salivation in five
rats. First few days showed serosanguineous lacrimation or nasal discharge and afterwards the rats appeared normal throughout the 14 day
observation period.

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: other: Study Director Approval

Conclusions:

The acute inhalation LC50 for 3030x18 is found to be greater than 164.4 mg/L.

Executive summary:

Ten male rats were exposed by whole body exposure to an atmosphere containing the test material as an airborne dust for an exposure period of one hour. Following the exposure process, animals were observed for 14 days, sacrificed, then a necroscopic examination performed.

There were no deaths during the study. Test substance residues were seen on the fur of most of the exposed rats. Serosanguineous lacrimation and nasal discharge was noted in occasional rats in first few days of the study. Followed by normal appearance for the remainder of the 14 day observation period. Gross necropsy revelaed no pathological alterations.

The1-hour LC50 was estimated to be in excess of 164.4 mg/L of air.