Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Liquid/Liquid extraction with dichloromethan

Test solutions

Vehicle:
yes
Details on test solutions:
5 mg/L with ethanol as vehicule

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
BREEDING CONDITIONS:
- culture in lab
- Temperature : 23 °C +/- 2 °C
- Brightness : continuous between 6000 and 10000 Lux
- Breeding environment

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
23 °C +/- 2 °C
pH:
8.1 +/- 0.2
Details on test conditions:
cintinuous light
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 282 µg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Results with reference substance (positive control):
1.36 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Fatty acid ethyl ester is non toxic to algea at solubility limit.