Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from october to november 2010
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Liquid/Liquid extraction with dichloromethan

Test solutions

Vehicle:
yes
Details on test solutions:
5 mg/L with ethanol as vehicule

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
BREEDING CONDITIONS:
- Temperature : 23°C +/- 2°C
- Brightness : day/night cycle 16h/8h
- freshwater

Study design

Test type:
semi-static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Test temperature:
23°C +/- 2°C
pH:
7.8 +/- 0.2
Dissolved oxygen:
> 60 %
Details on test conditions:
Photoperiod 16/8
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
> 282 µg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
Between 200 and 400mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Fatty acid ethyl ester is non toxic to fish at solubility limit.