3-Oxabicyclo[3.1.0]hexan-2-one, 4-(1,1-dichloro-2,2,2-trifluoroethyl)-6,6-dimethyl-, (1R,4R,5S)-

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(1R,4R,5S)- 4-(1,1-Dichloro-2,2,2-trifluoroethyl)-6,6-dimethyl-3-oxabicyclo[3.1.0]hexan-2-one

EC Number:

606-114-2

Cas Number:

187592-70-7

Molecular formula:

C9H9Cl2F3O2

IUPAC Name:

(1R,4R,5S)- 4-(1,1-Dichloro-2,2,2-trifluoroethyl)-6,6-dimethyl-3-oxabicyclo[3.1.0]hexan-2-one

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

White powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The actual exposure concentration of the test product were determined by the analytical laboratory. Duplicate samples of the specially prepared test flasks at each sampling time (24h; 48h and 72H) were stored at -20°C±2.0°C until analyses.

Test solutions

Vehicle:

no

Details on test solutions:

In the stock solution of 46.5 mg/l a white precipitate was found soring in the volumetric flask. In order to verify the actual concentration the stock solution was filtered and the filtrate was analysed at Cheminova. The result of the analysis was 44 mg/l.

Test organisms

Test organisms (species):

Skeletonema costatum

Details on test organisms:

NIVA BAC 1

Study design

Water media type:

saltwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

18.5-19.5°C

pH:

8

Salinity:

28 ‰

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 37.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 37.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

18.9 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

biomass

Details on results:

pH in control vessels deviated > 1 pH unit (1.2) but this is assessed not to have influence on the result.

Results with reference substance (positive control):

- Results with reference substance valid: Yes
- EC50: 1.06 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Cell density increased by a factor of more than 16 during test, results for reference substance within acceptable range, 1.0-2.20 mg/l %

Conclusions:

EC50(72 h) of Fluorolactone, CAS No. 187592-70-7 is 18.9 mg/L for Skelotonema costatum.