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EC number: -
CAS number: -
Exponentially growing cultures of a unicellular freshwater green alga Desmodesmus subspicatus were exposed to different concentrations of 400160 for a period of 72h. Under the tested conditions the lowest concentration which causes reduction of the growth compared to the control within a 72-h exposure period was 100 mg/L (=LOELR) whereas the highest concentration not causing growth inhibition (=NOEC) was 46 mg/L. Therefore the loading rate of the test substance causing 50% reduction in growth was higher than 100 mg/L (=EL50).
has been performed according to OECD guideline 201 and EU method C.3 in
a GLP certified testing facility.
was performed using five concentrations of 400160 ranging from 4.6 to
100 mg/L. The unicellular freshwater green alga Desmodesmus subspicatus
was exposed for a period of 72 h. The cell concentration of each
replicate was determined by measuring the absorption of the cuvettes at
440 nm every 24 h with a spectral photometer. With the absorption
values, the cell number was calculated. Then the growth rate µ, the area
under the growth curve (AUC) and the yield were determined.
As the test
item is poorly soluble in water, the water-accommodated fraction was
tested. Results based on the loading rate (nominal concentrations) as
the measured concentrations of 400160 in medium were lower than the
limit of quantification.
dichromate was tested as positive control. No observations were made
which might cause doubts concerning the validity of the study outcome as
all validity criteria were met. Therefore the results of the test can be
The following parameters were
Growth rate (µ)
> 100 mg/L
Approx. 100 mg/L
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