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EC number: 267-021-7 | CAS number: 67762-52-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 Jul - 12 Aug 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.20 (Daphnia magna Reproduction Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1300 (Daphnid Chronic Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Department of Health, United Kingdom
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 1, 10, 100 mg/L
- Sampling method: Samples were taken after 0, 3 (old), 5 (fresh), 7 (old), 10 (fresh), 12 (old), 19 (fresh) and 21 (old) d - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Nominal amounts of test item (5.0, 50 and 500 mg) were each separately added to the surface of 5 liters of test water to give the 1.0, 10 and 100 mg/L loading rates respectively. After the addition of the test item, the test water was stirred by magnetic stirrer using a stirring rate such that a vortex was formed to give a dimple at the water surface. The stirring was stopped after 95 hours and the mixtures allowed to stand for 1 hour. A wide bore glass tube, covered at one end with Nescofilm was submerged into the vessel, sealed end down, to a depth of approximately 5 cm from the bottom of the vessel. A length of Tygon tubing was inserted into the glass tube and pushed through the Nescofilm seal. Microscopic inspection of the WAFs showed no micro-dispersions or undissolved test item to be present. The aqueous phase or WAF was removed by mid-depth siphoning (the first approximate 75-100 mL discarded) to give the 1.0, 10 and 100 mg/L loading rate WAFs.
- Eluate: no
- Differential loading: yes
- Controls: yes, test medium control - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: in-house culture
- Age of parental stock (mean and range, SD): 1st instar Daphnia magna
- Feeding during test: Each daphnid received approximately 5 to 10 μL of an algal suspension (Desmodesmus subspicatus) and approximately 20 μL of Tetramin® flake food suspension daily. Feeding was at a level of approximately 0.1 to 0.2 mg carbon/daphnid/day, dependent on the age and size of the animals. Equal amounts of food were given to each daphnid.
- Culture conditions: Adult daphnia were maintained in 150 mL glass beakers containing Elendt M7 mediumat approximately 20 °C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods. Each culture was fed daily with a mixture of algal suspension (Desmodesmus subspicatus) and Tetramin® flake food suspension. Culture conditions ensured that reproduction was by parthenogenesis. Gravid adults were isolated the day before initiation of the test, such that the young daphnids produced overnight were less than 24 hours old. These young were removed from the cultures and used for testing. The diet and diluent water are considered not to contain any contaminant that would affect the integrity or outcome of the study. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 248 - 268 mg/L as CaCO3
- Test temperature:
- 21 - 22 °C
- pH:
- 7.6 - 8.1
- Dissolved oxygen:
- 8.0 - 9.0 mg O2/L
- Nominal and measured concentrations:
- nominal: control, 1, 10, 100 mg/L
measured control: < LOQ
measured 1 mg/L: < LOQ
measured 10 mg/L: 0.0202 mg/L (3 d), 0.0190 mg/L (5 d), < LOQ (7 d), 0.0181 mg/L (day 10), < LOQ (day 12), < LOQ (day 19), < LOQ (day 21)
measured 100 mg/L: 0.0240 mg/L (3 d), 0.0269 mg/L (5 d), 0.0233 mg/L (7 d), 0.0199 mg/L (day 10), < LOQ (day 12), 0.00780/0.00905 mg/L (day 19, duplicate samples), < LOQ (day 21, duplicate samples) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 mL glass vessels
- Type (delete if not applicable): closed with a plastic lid
- Material, size, headspace, fill volume: glass, 150 mL, headspace: 50 mL, fill volume: 100 mL
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): renewed 3 times a week on days 3, 5, 7, 10, 12, 14, 17 and 19.
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark with 20 min transition period
- Light intensity: 592 - 718 lux
EFFECT PARAMETERS MEASURED
- Mobility and length of the parental generation, numbers of live and dead F1 daphnia and the number of discarded unhatched eggs was counted every day. - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- EL10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOELR
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of parent animals: 0%
- No. of offspring produced per day per female: 99.1 (control), 105.9 (1 mg/L), 103.4 (10 mg/L), 101.7 (100 mg/L)
- Body length and weight of parent animals: After 21 days the length of each surviving adult was determined. The results showed that there were no statistically significant differences (P≥ 0.05) between the control and the 1.0, 10 and 100 mg/L loading rate WAF test groups in terms of length of the daphnids after 21 days exposure to the test item.
- Visual observation of test solutions: clear and colorless (microscopic observation of the WAF)
- Time to first brood release or time to hatch: 7-9 d - Reported statistics and error estimates:
- Results from the control and the each test group were compared using one way analysis of variance incorporating Bartlett's test for homogeneity of variance (Sokal and Rohlf 1981) and the Williams test for differences between treatment means when several dose levels are compared with a zero dose control (Williams 1971).
Reference
Table 1: Total cumulative production of live young
Nominal loading rate [mg/L] |
Day |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
7 |
87 |
126 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
106 |
159 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
9 |
113 |
141 |
100 |
0 |
0 |
0 |
0 |
0 |
0 |
10 |
15 |
103 |
139 |
Nominal loading rate [mg/L] |
Day |
||||||||||
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
|
Control |
123 |
271 |
323 |
323 |
462 |
504 |
506 |
751 |
775 |
775 |
991 |
1 |
159 |
373 |
396 |
396 |
535 |
553 |
591 |
837 |
858 |
858 |
1059 |
10 |
141 |
299 |
326 |
326 |
480 |
521 |
527 |
764 |
811 |
811 |
1034 |
100 |
139 |
209 |
320 |
342 |
498 |
520 |
543 |
746 |
795 |
795 |
1017 |
Table 2: Body length of surviving adults at day 21
Nominal loading rate [mg/L] |
Individual daphnia lengths [mm] |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
|
Control |
3.9 |
4.0 |
3.9 |
3.9 |
3.9 |
4.2 |
3.8 |
3.5 |
3.9 |
3.9 |
1 |
3.9 |
3.7 |
4.1 |
3.8 |
4.0 |
3.9 |
3.8 |
3.9 |
4.0 |
4.0 |
10 |
3.9 |
4.0 |
4.0 |
3.9 |
4.0 |
3.8 |
3.8 |
3.8 |
3.9 |
3.9 |
100 |
3.9 |
3.5 |
3.7 |
4.1 |
3.9 |
3.9 |
3.9 |
3.6 |
3.8 |
3.8 |
Description of key information
Key value for chemical safety assessment
Additional information
The long-term toxicity of Carboxylic acids, C5-9, hexaesters with dipentaerythritol (CAS 67762-52-1) was investigated in a semi-static test following OECD 211. Daphnia magna were exposed to nominal exposure concentrations of 1, 10 and 100 mg/L for 21 days. Significant effects on the survival and reproduction rate were not determined.
A NOEC ≥ 100 mg/L (nominal) based on the reproduction rate was determined.
Chronic effects of the substance on aquatic organisms are not expected.
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