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Ecotoxicological information

Toxicity to soil microorganisms

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No experimental data investigating the effects on soil microorganisms are available for 1,2,3-propanetriol, homopolymer, diisooctadecanoate (CAS 63705-03-3). Therefore, all available related data is combined in a Weight of Evidence (WoE) approach, which is in accordance to the REACh Regulation (EC) No 1907/2006, Annex XI, 1.2, to adapt the data requirements of Regulation (EC) No 1907/2006 Annex VII - X (ECHA guidance section R.7.11.5.3).

The test substance is characterized by a high log Koc (log Koc >= 2.95) indicating a potential for adsorption to the soil particles. Tests with soil-dwelling organisms that feed on soil particles are therefore most relevant for the evaluation of soil toxicity of 1,2,3 -propanetriol, homopolymer, diisooctadecanoate. In the absence of a clear indication of selective toxicity, an invertebrate (earthworm or collembolan) test is preferred, as outlined in ECHA guidance section R.7.11.5.3 (ECHA, 2012). Chronic soil macroorganism data are available for 1,2,3 -propanetriol, homopolymer, diisooctadecanoate showing effects on reproduction for earthworms in a chronic test according to OECD 222 (NOEC (56 d) = 250 mg/kg soil dw). Moreover, 1,2,3 -propanetriol, homopolymer, diisooctadecanoate is readily biodegradable and no inhibition of the respiration rate was observed after 3 h for activated sludge microorganisms in a test according to OECD 209 (EC10/EC50 (3 h) > 1000 mg/L). The applied test concentration is higher than concentrations that can be expected in the influent of a sewage treatment plant, as the substance has low water solubility (< 0.15 mg/L). The ECHA Guidance Document R.7c (ECHA, 2012) states that a test on soil microbial activity will only be additionally necessary for a valid PNEC derivation if inhibition of sewage sludge microbial activity has occurred.

This is supported by further evidence from literature data. This data showed that soil microorganism communities are well capable of degrading fatty acid esters (Hita et al., 1996 and Cecutti et al., 2002) and use them as energy source (Banchio&Gramajo, 1997). Hita et al. investigated the degradation of the model molecule tristearin which is a triglyceride containing of glycerin tri-esterified with stearic acid in three different soils for 4 weeks. The amount of stearic acid increased in considerable amounts during the experiment showing the hydrolytic activity of lipases breaking the ester bonds. The investigation of ester fractions moreover showed the generation of new alkanoic acids (methyl stearate, ethyl stearate and propyl stearate) which were not determined in the controls. Nevertheless the amounts were no longer present after 4 weeks, which leads to the assumption that degradation by soil microorganisms had occurred. The same was shown by Cecutti et al. One soil sample was chosen and incubated with methyl oleate (plant oil) for 120 d. Methyl oleate and its metabolites were completely degraded after 60 d. Streptomyces coelicolor, a common gram-positive soil bacterium uses fatty acids (C4-C18) as sole carbon end energy source indicating that fatty acids are not-toxic and can be used for catabolism (Banchio and Gramajo, 1997). The available literature data shows that soil microorganisms are capable to break-up ester bonds and degrade fatty acids in significant amounts. Moreover, the data indicated the non-toxic properties of fatty acids since they can be used as energy source.

Taking all the available information into account in a Weight of Evidence approach in accordance with Annex XI, 1.2, effects on soil microorganisms are thus not expected to be of concern, and consequently, no further testing is required.