Registration Dossier
Registration Dossier
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EC number: 200-385-7 | CAS number: 58-55-9
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
the inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.
Key value for chemical safety assessment
Additional information
Two studies on toxicity of caffeine to microorganisms are available.
In a guideline study according to German standard DIN 38412, part 8, the toxicity of theophylline to Pseudomonas putida was investigated. In a 17 hour test the growth rate of P. putida in treatments with nominal concentrations ranging from 39.06 to 5000 was determined and compared to the growth rate of the bacterium in a control. The EC50 for P. putida was 2110 mg/L.
In a 180 min respiration inhibition test according to OECD guideline 209, the effect of theophylline on the respiration rate of activated sludge taken from a waste water treatment plant treating domestic sewage was investigated. The EC50 (180 min) for theophylline was 900 mg/L.
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