Heptanal, 2-(phenylmethylene)-, (2E)-

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The experiment was done according to the standard method of in vivo micronucleus. However, in the article there is no mention of GLP status and positive control.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1983

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)

Deviations:

yes

Remarks:

. No positive control, only one sampling time (30hr)

GLP compliance:

not specified

Type of assay:

micronucleus assay

Test material

Test material information

Constituent 1

Reference substance name:

Hexylcinnamaldehyde

IUPAC Name:

Hexylcinnamaldehyde

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: purchased from Ivanovas GmbH, Kisslegg
- Age at study initiation: 10-14 week old
- Weight at study initiation: Not reported
- Assigned to test groups randomly: [no/yes, under following basis: ] Not reported
- Fasting period before study: Not applicable
- Housing: Not reported
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: Not reported

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

- Vehicle(s)/solvent(s) used: Olive oil

Details on exposure:

The dose was given by i.p. injection once, using olive oil as a vehicle. Eight animals were used for each dose group.

Duration of treatment / exposure:

The mice were killed and bone-marrow smears were prepared 30 hr after treatment.

Frequency of treatment:

Single injection

Post exposure period:

The mice were killed and bone-marrow smears were prepared 30 hr after treatment.

Doses / concentrations

Remarks:

Doses / Concentrations:
0 (olive oil), 324, 540, 756 mg/kg bw
Basis:
actual ingested

No. of animals per sex per dose:

Eight

Control animals:

yes, concurrent vehicle

Positive control(s):

Not reported

Examinations

Tissues and cell types examined:

The mice were killed and bone-marrow smears were prepared 30 hr after treatment.

Details of tissue and slide preparation:

No further details available

Evaluation criteria:

If significantly different from concurrent vehicle control, the result was judged as positive.

Statistics:

Statistical significance was determined according to the methods of Kastenbaum & Bowman (1970).

Results and discussion

Test results

Sex:

male/female

Genotoxicity:

negative

Toxicity:

not specified

Vehicle controls validity:

valid

Negative controls validity:

not applicable

Positive controls validity:

not specified

Any other information on results incl. tables

  

Dose (mg/kg)	Surviving/treated mice	Mean no. of micronucleated PE/1000 PE
1 x 756	8/8	2.4
1 x 540	8/8	1.8
1 x 324	8/8	2.1
0	8/8	1.0

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): negative
Exposure to HCA did not result in an increase in the proportion of micronucleated PCEs.

Executive summary:

In an in vivo micronucleus assay performed similarly to the OECD guideline No. 474 with minor deviations, male/female NMRI mice were exposed to alpha-Hexylcinnamaldehyde (HCA) diluted in olive oil by a single intraperitoneal dose. Different dose levels (324, 540, 756 mg/kg bw) were tested (8 animals per dose). 30 hours after the injection, the bone marrow cells were collected and one thousand polychromatic erythrocytes were observed in order to quantify the number of micronucleated polychromatic erythrocytes. Concurrent vehicle animals were used as negative control, no data was available on positive control. Under the test conditions, there was no significant increase of micronucleated polychromatic erythrocytes, if compared with the concurrent control. No mortality was observed. Therefore, under the study conditions it is concluded that HCA is not clastogenic.