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Diss Factsheets
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EC number: 701-373-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 11, 2012 to august 9, 2012
- Reliability:
- 1 (reliable without restriction)
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: ISO Standard 10634
- Qualifier:
- according to guideline
- Guideline:
- other: ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)
- Qualifier:
- according to guideline
- Guideline:
- other: EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 301
- Deviations:
- no
- GLP compliance:
- yes
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- -Source: The source of test organisms was activated sludge freshly obtained from a municipal
sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
-Treatment: The freshly obtained sludge was kept under continuous aeration until further treatment. The concentration of suspended solids was 2.9 g/l in the concentrated sludge (information obtained from the municipal sewage treatment plant). Before use, the sludge was allowed to settle (50 minutes) and the supernatant liquid was used as inoculum at the amount of 10 ml/l of mineral medium. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 28 d
- Test temperature:
- 22.1 and 22.7 °C
- pH:
- At t=0 d: 7.6
At t=28 d: 7.5 – 7.8 - Details on test conditions:
- - Test temperature: between 22.1 and 22.7°C.
- pH:
At t=0 d: 7.6
At t=28 d: 7.5 – 7.8
- pH adjusted: no
- Aeration of dilution water: Not before the test, the test is aerated continously
- Suspended solids concentration: The concentration of suspended solids was 2.9 g/l in the c
oncentrated sludge (information obtained from the municipal sewage treatment plant). Before use, the sludge was allowed to settle (50 minutes) and the liquid was decanted for use as inoculum at the amount of 10 ml/l of mineral medium.
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 2 litre all-glass brown coloured bottles
- Number of culture flasks/concentration:
Test suspension: containing test substance and inoculum (2 bottles).
Inoculum blank: containing only inoculum (2 bottles)
Positive control: containing reference substance and inoculum (1 bottle).
Toxicity control: containing test substance, reference substance and inoculum (1 bottle).
- Method used to create aerobic conditions:
Synthetic air (a mixture of oxygen (ca. 20%) and nitrogen (ca. 80%)) was sparged through the s
olutions at a rate of approximately 1-2 bubbles per second (ca. 30-100 ml/min).
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used:
CO2 was trapped in barium hydroxide solution. The amount of CO2 produced was determined by
titrating the remaining Ba(OH)2 with 0.05 M standardized HCl (1:20 dilution from 1 M HCl (Titrisol®ampul). Titrations were made on days 2, 5, 9, 14, 19, 23, 27 and 29 for the inoculum blank. For the positive and toxicity control, tritrations were made over a period of 14 days.
SAMPLING
- Sampling frequency: Titration were made on day: 2, 5, 9, 14, 19, 23, 27 and 29
- Sampling method: Titration of the whole volume of CO2-absorber
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes
- Other: Positive control containing reference substance and inoculum - Reference substance (positive control):
- yes
- Remarks:
- sodium acetate
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 12 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- other: CO2 evolution
- Remarks on result:
- other: Based on DOC
- Details on results:
- In the toxicity control more than 25% biodegradation occurred within 14 days (36%, based on
ThCO2). Therefore, the test substance was assumed not to inhibit microbial activity. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions RD04/201 was not readily biodegradable under modified Sturm test.
- Executive summary:
A study was conducted to determine the ready biodegradability of the substance using the CO2 evolution test according to OECD Guideline 301B, EU Method C.4C and ISO DIS Method 9439, under GLP principles. A single test concentration of 12 mg/L (as total organic carbon, TOC) was tested for 28 days. The study consisted of two replicates of inoculum blanks, two replicates of the test bottles and a single bottle for the reference substance (sodium acetate) and a single bottle of the toxicity control. The test solutions were continuously stirred during the test, to ensure optimal contact between the test substance and the test organisms. The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of the test substance. In the toxicity control, the test substance was not found to inhibit microbial activity. The study met all acceptability criteria stated in the OECD guideline. Under the study conditions, the NOEC of the test substance to activated sludge microorganisms was determined to be 12 mg/L (Desmares-Koopmans MJE, 2012).
Reference
Description of key information
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 12 mg/L
Additional information
A study was conducted to determine the ready biodegradability of the substance using the CO2 evolution test according to OECD Guideline 301B, EU Method C.4C and ISO DIS Method 9439, under GLP principles. A single test concentration of 12 mg/L (as total organic carbon, TOC) was tested for 28 days. The study consisted of two replicates of inoculum blanks, two replicates of the test bottles and a single bottle for the reference substance (sodium acetate) and a single bottle of the toxicity control. The test solutions were continuously stirred during the test, to ensure optimal contact between the test substance and the test organisms. The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of the test substance. In the toxicity control, the test substance was not found to inhibit microbial activity. The study met all acceptability criteria stated in the OECD guideline. Under the study conditions, the NOEC of the test substance to activated sludge microorganisms was determined to be 12 mg/L (Desmares-Koopmans MJE, 2012).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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