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EC number: 860-695-9 | CAS number: 88247-41-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 Dec 2019 - 12 Feb 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N,N-bis(2-ethylhexyl)formamide
- EC Number:
- 860-695-9
- Cas Number:
- 88247-41-0
- Molecular formula:
- C17H35NO
- IUPAC Name:
- N,N-bis(2-ethylhexyl)formamide
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Standard activated sludge obtained from the Chemicals Evaluation and Research Institute, Japan
- Date of receipt: November 21, 2019
- Concentration of sludge: 2300 mg/L mixed liqor suspended solids (MLSS)
- Initial cell/biomass concentration: inoculum concentration = 30 mg/L
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Parameter followed for biodegradation estimation:
- O2 consumption
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Standard mineral medium according to OECD guideline
- Test temperature: 25 +/- 1 °C
- pH: 7 +/- 0.04
- pH adjusted: yes
- Suspended solids concentration: 2300 mg/L mixed liquor suspended solids (MLSS)
TEST SYSTEM
- Culturing apparatus: Closed system apparatus for measuring oxygen consumption, OM-3100A, 300 mL glass bottles
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: continuous stirring with magnetic stirrer
- Measuring equipment: BOD measurements apparatus, TOC analyzer, GC for analytical determination of residual test substance, pH meter
- Details of trap for CO2 and volatile organics if used: soda lime
SAMPLING
- Sampling frequency: at start of the BOD measurement pH values were measured in the inoculum blank and abiotic control, at the end of BOD measurements pH was measured in all test solutions; DOC measurement was conducted in abiotic control, test solutions, and inoculum blank after the end of the BOD measurement at day 28;
- Sampling method: For DOC measurement 12 mL of test solution were sampled
CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Abiotic sterile control: 1
- Reference control: 1
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other:
- Remarks:
- results based on BOD
- Parameter:
- % degradation (test mat. analysis)
- Value:
- >= 0 - <= 1
- Sampling time:
- 28 d
- Remarks on result:
- other:
- Remarks:
- The degradability based on residual analysis of the test material was 0, 1, and 1% in the three samples
- Details on results:
- The degradability based on DOC after 28 days was not calculated since the test substance was insoluble in water and the DOC in the abiotic blank after 28 days was less than 90% of the theoretical value.
Since the biodegradation based on residual analysis of the test substance showed approximately 1% degradation and no additional peak was detected on the GC chromatogram, it was conluded that the test substance was not transformed.
BOD5 / COD results
- Results with reference substance:
- The biodegradation based on BOD measurements was 60% after 7 days and 85% after 28 days for the reference substance aniline.
Any other information on results incl. tables
Table 1. Measured BOD and pH values
Bottle No. |
Sample |
BOD (mgO2) |
pH |
||||
Day 7 |
Day 14 |
Day 21 |
Day 28 |
Day 0 |
Day 28 |
||
1 |
Abiotic control |
0.1 |
0.1 |
0.1 |
0.1 |
7.7 |
7.7 |
2 |
Test item 1 |
2.0 |
4.1 |
4.1 |
4.1 |
- |
7.2 |
3 |
Test item 2 |
2.3 |
4.6 |
4.7 |
4.7 |
- |
7.2 |
4 |
Test item 3 |
2.5 |
4.6 |
4.7 |
4.7 |
- |
7.1 |
5 |
Reference control |
50.0 |
73.2 |
75.7 |
78.8 |
- |
7.9 |
6 |
Inoculum blank |
6.5 |
11.8 |
12.2 |
12.5 |
7.0 |
7.3 |
Table 2. Corrected measured values at day 28
|
Test item |
Abiotic control |
Theoretical value |
||
|
Bottle No. 2 |
Bottle No. 3 |
Bottle No. 4 |
||
BOD (mgO2)* |
- 8.4 |
- 7.8 |
-7.8 |
0.1 |
94.4 |
DOC* |
0.1 |
0.2 |
0.4 |
0.4 |
22.7 |
Residue analysis (mg) |
30.9 |
30.4 |
30.5 |
30.8 |
30.0 |
Residue analysis (%) |
103 |
101 |
102 |
103 |
- |
* values of the test item were corrected for BOD or DOC values of the inoculum blank
Table 3. Biodegradation (%) at day 28
|
Test item |
Average |
||
|
Bottle No. 2 |
Bottle No. 3 |
Bottle No. 4 |
|
BOD |
0 (-9) |
0 (-8) |
0 (-8) |
0 |
DOC |
NA |
NA |
NA |
- |
Residue analysis |
0 |
1 |
1 |
1 |
NA = not available, due to insolubility of test item in water (< 90% DOC of test item in abiotic control)
Table 4. Validity criteria for OECD 301C
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg O2/L in 28 days. Values higher than 60 mg/L require critical examination of the data and the experimental technique. If the pH value is outside the range 6-8.5 and the oxygen consumption by the test substance is less than 60%, the test could be repeated with a lower concentration of test substance. |
- Oxygen uptake based on BOD was 41.67 mgO2/L at day 28 (12.5 mgO2/0.3 L = 41.67 mgO2/L) - pH was between 7.0 – 7.9 in all vessels
|
Yes |
A test is considered valid if the difference of extremes of replicate values of the removal of the test substance at the plateau or at the end of the test, as appropriate is less than 20% and if the percentage degradation of aniline calculated from the oxygen consumption exceeds 40% after 7 days and 65% after 14 days. |
- Differences of extremes of replicate values of biodegradation of test substance = 7% based on corrected BOD - Degradation of aniline at 7 days = 60% and at 28 days = 85% |
Yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 4 in 'any other information on results incl. tables'
- Interpretation of results:
- not readily biodegradable
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