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EC number: 948-383-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 28 - 29 Oct 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Department of Health, UK
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Information provided by the Sponsor indicated that the test substance is not sufficiently soluble to prepare an aqueous solution. Therefore, at test inition weights were established by the addition of appropriate volumes (based on specific gravity, 0.99) of test substance to the test vessels containing RO water, synthetic sewage and microbial inoculum (see Table 1). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: A sample of activated sludge was obtained the day before the start of the test from Worlingworth Sewage Treatment Works, which treats predominantely domestic waste. In the laboratory, the sample was maintained under aerobic conditions until required. One day one of collection, an aliquot (10 mL) of the activated sludge was filtered through a dried, preweighed Whatman GF/C filter paper, which was then dried again at ~ 105 °C for at least one hour, allowed to cool in a desiccator before being reweighed. The mixed liquor suspended solids content of the activated sludge was then calculated. Synthetic sewage (50 mL/L) was added to the stock of activated sludge. The mixture was aerated overnight. The mixed liquor suspended solids content of the activated sludge was adjusted to 4 g/L by addition of tap water. Aliquots (200 mL) were added to the test vessels to give a final suspended solids concentration of 1.6 g/L.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- test start: 19.5 - 19.6 °C
test end: 19.3 - 19.4 °C - pH:
- test start: 7.3 - 7.4
test end: 7.6 - 7.9 - Nominal and measured concentrations:
- Nominal: 10, 100 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Type: closed (loosely covered with aluminium foil)
- Aeration: using a glass aerator connected to a laboratory supply of oil-free compressed air (~ 1 L/min.)
- No. of vessels per concentration: 1 (10, 100 mg/L); 3 (1000 mg/L)
- No. of vessels per control: 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reverse osmosis (RO) water
- Culture medium different from test medium: no, according to OECD guideline 209
- Intervals of water quality measurement: The pH and temperature were measured at the start and end of the test.
EFFECT PARAMETERS MEASURED: The respiration rate was measured after a test period of 3 h. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The test substance showed no inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the test. Respiration rates of mixtures containing the test substance showed an increase in respiration rate above that of the controls.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (3h) = 6.4 mg/L (4.1 - 9.6 mg/L) - Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the analogue justification attached to IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Conclusions:
- The key values for the toxicity to activated sluge microorganisms are a EC10 (3 h) of > 1000 mg/L (nominal), based on experimental data with one structurally and chemically closely related source substances (OECD 209, activated sludge).
Referenceopen allclose all
Table 1: Preparation of test solutions
Treatment |
Test substance [µL] or reference substance [mL] |
Synthetic sewage [mL] |
RO water [mL] |
Microbial Inoculum [mL] |
Control 1 |
0 |
16 |
284 |
200 |
Control 2 |
0 |
16 |
284 |
200 |
Test substance [mg/L] |
|
|
|
|
10 |
5.1 |
16 |
284 |
200 |
100 |
50 |
16 |
284 |
200 |
1000 |
505 |
16 |
284 |
200 |
Reference substance [mg/L] |
|
|
|
|
3 |
3 |
16 |
281 |
200 |
10 |
10 |
16 |
274 |
200 |
32 |
32 |
16 |
252 |
200 |
Table 2: Dissolved oxygen concentration and measurement times
Treatment |
Initial dissolved oxygen conc. in culture [mg O2/L] |
Initial measured dissolved oxygen conc. [mg O2/L] |
Final measured dissolved oxygen conc. [mg O2/L] |
Measurement time [min] |
Control 1 |
6.9 |
6.5 |
2.5 |
8.5 |
Control 2 |
6.9 |
6.5 |
2.5 |
9.0 |
Test substance [mg/L] |
|
|
|
|
10 |
4.5 |
4.2 |
1.5 |
5.3 |
100 |
5.0 |
4.8 |
1.5 |
6.3 |
1000 |
5.8 |
5.5 |
2.5 |
5.7 |
1000 |
4.9 |
4.6 |
1.5 |
5.8 |
1000 |
5.5 |
4.9 |
2.0 |
5.8 |
Reference substance [mg/L] |
|
|
|
|
3 |
7.0 |
6.5 |
3.2 |
9.5 |
10 |
8.0 |
7.8 |
5.8 |
9.9 |
32 |
7.4 |
8.1 |
7.7 |
8.1 |
Table 3: Measurement of respiration rate
Treatment |
Specific respiration rate [mg O2/g/h] |
% inhibition or stimulation () |
Control 1 |
17.6 |
- |
Control 2 |
16.7 |
- |
Test substance [mg/L] |
|
|
10 |
19.1 |
0 (11) |
100 |
19.6 |
0 (14) |
1000 |
19.7 |
0 (15) |
1000 |
20.0 |
0 (17) |
1000 |
18.8 |
0 (9) |
Reference substance [mg/L] |
|
|
3 |
13.0 |
24 |
10 |
7.6 |
56 |
32 |
1.9 |
89 |
Description of key information
Key value for chemical safety assessment
Additional information
Since no studies investigating the toxicity of Fatty acids, C8-10-(even numbered), esters with pentaerythritol and adipic acid (EC 948-383-1) to microorganisms are available, in accordance with Regulation (EC) No 1907/2006 Annex XI, 1.5, a read-across to the structurally related source substance Decanoic acid, mixed esters with heptanoic acid, octanoic acid, pentaerythritol and valeric acid (CAS 71010-76-9) was conducted. The source substance is representative to evaluate the toxicity of the target substance to microorganisms. This read-across is justified in detail within the analogue justification in IUCLID section 13.
The study with the source substance Decanoic acid, mixed esters with heptanoic acid, octanoic acid, pentaerythritol and valeric acid (CAS 71010-76-9) was conducted under GLP in a static test design according OECD Guideline 209 (Respiration Inhibition Test), using activated sludge from predominantly domestic sewage. Three nominal concentrations of 10, 100 and 1000 mg/L of the test substance were prepared. The respiration rate was measured after a test period of 3 h. The test substance showed no inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the test. Therefore, effect values of EC50 (3 h) > 1000 mg/L (nominal) and EC10 (3 h) > 1000 mg/L (nominal) were determined.
Based on this result obtained from the source substance Decanoic acid, mixed esters with heptanoic acid, octanoic acid, pentaerythritol and valeric acid (CAS 71010-76-9) (in accordance with Regulation (EC) No 1907/2006 Annex XI, 1.5) which is characterized by a similar ecotoxicological profile and comparable structure, it can be concluded that the target substance Fatty acids, C8-10-(even numbered), esters with pentaerythritol and adipic acid (EC 948-383-1) is non-inhibitory of aquatic microorganisms activity and further testing for this endpoint is regarded not necessary.
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