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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20 July 2010 to 4 August 2010
Reliability:
1 (reliable without restriction)
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Analytical monitoring:
yes
Details on sampling:
Frequency at t=0 h and t=48 h
Volume 2 and 4.9 ml from the approximate centre of the test vessels
Storage Samples were stored in a freezer until analysis.

At the end of the exposure period, the replicates were pooled at each concentration before sampling.
Vehicle:
no
Details on test solutions:
The standard test procedures required generation of test solutions, which should contain completely dissolved test substance concentrations or stable and homogeneous mixtures or dispersions. The testing of concentrations that disturb the test system should be prevented (e.g. film of the test substance on the water surface).

The batch of MEA Polyborate 1:1 tested was a clear colourless liquid composed of 40% of salt in water and completely soluble in test medium at the concentrations tested.

Preparation started with a concentration of 1000 mg active ingredient (a.i.)/l which corresponds to 2500 mg MEA Polyborate 1:1 per litre. To this end 1.95 ml of the test substance was added to 1000 ml test medium. No other treatment than vigorous shaking was needed to completely dissolve the test substance. The pH of the highest concentration was adjusted from 9.6 to 8.0 in the range-finding test and from 9.8 to 7.9 in the final test with 1M HCl (Merck, Darmstad, Germany). The lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. The final test solutions were all clear and colourless.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus 1820
- Source: In-house laboratory culture with a known history.
- Age at study initiation (mean and range, SD): For the test selection of young daphnids with an age of < 24 hours, from parental daphnids of more than two weeks old.
- Method of breeding:
- Feeding during test: Daily, a suspension of fresh water algae.

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
180 mg/L expressed as CaCO3
Test temperature:
18-22°C
pH:
7.8 - 8.0
Dissolved oxygen:
9.0 - 9.2 mg/L
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal: 100, 180, 320, 560, 1000 mg/L
Measured: 250, 450, 800, 1400, 2500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 ml, all glass
- No. of organisms per vessel: 5 per vessel containing 80 ml of test solution

Composition of medium:
Composition of medium M7:
Adjusted ISO medium: the following chemicals (analytical grade) are dissolved in tap water purified by Reverse Osmosis (RO-water, GEON Waterbehandeling, Berkel-Enschot, The Netherlands):
Macro salts: CaCl2.2H2O 211.5 mg/l
MgSO4.7H2O 88.8 mg/l
NaHCO3 46.7 mg/l
KCl 4.2 mg/l
Medium M7: trace elements, macro nutrients and vitamins are added to freshly prepared ISO medium to reach the following concentrations:
Trace elements:B 0.125 mg/l
Fe 0.05 mg/l
Mn 0.025 mg/l
Li, Rb and Sr 0.0125 mg/l
Mo 0.0063 mg/l
Br 0.0025 mg/l
Cu 0.0016 mg/l
Zn 0.0063 mg/l
Co and I 0.0025 mg/l
Se 0.0010 mg/l
V 0.0003 mg/l
Na2EDTA.2H2O 2.5 mg/l

Macro nutrients:Na2SiO3. 9H2O 10.0 mg/l
NaNO3 0.27 mg/l
KH2PO4 0.14 mg/l
K2HPO4 0.18 mg/l

Vitamins: Thiamine 75.0 µg/l
B12 1.0 µg/l
Biotin 0.75 µg/l
Reference substance (positive control):
not specified
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
496 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 457 - 563 mg/l
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
423 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 320 - 560 mg/l
Details on results:
- Mortality of control: 0
- Abnormal responses: Not reported
Validity criteria fulfilled:
yes
Remarks:
0% in controls
Conclusions:
Under the conditions of a GLP OECD 202 study MEA Polyborate 1:1 did not induce acute immobilisation of Daphnia magna at 320 mg a.i./l after 48 hours of exposure (NOEC).

The 48h-EC50 was 423 mg a.i./l based on nominal concentrations (95% confidence interval between 320 and 560 mg a.i./l).
Executive summary:

Acute Toxicity Study inDaphnia magnawith MEA Polyborate 1:1.

 

The study procedures described in this report were based on the OECD guideline No. 202, 2004. In addition, the procedures were designed to meet the test methods of theCommissionRegulation (EC) No440/2008,Part C.2, 2008 and the ISO International Standard 6341, 1996.

 

The batch of MEA Polyborate 1:1 tested was a clear colourless liquid composed of 40% of salt in water and completely soluble in test medium at the concentrations tested.

 

Preparation started with a concentration of 1000 mg active ingredient (a.i.)/l which corresponds to 2500 mg MEA Polyborate 1:1 per litre. To this end 1.95 ml of the test substance was added to 1000 ml test medium. No other treatment than vigorous shaking was needed to completely dissolve the test substance. The pH of the highest concentration was adjusted with 1M HCl.

The lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium.

 

A final test was performed based on the results of a range-finding test. Twenty daphnids per group (5 per replicate, 4 replicates) were exposed to a control and concentrations of 100, 180, 320, 560 and 1000 mg a.i./l. The total test exposure time was 48 hours and samples for the analytical confirmation of the exposure concentrations were taken at the start and at the end of the test.

 

Analyses were performed on samples taken form the nominal concentrations of 800, 1400 and 2500 mg/l (i.e. 320, 560 and 1000 mg a.i./l). The concentrations of both boron and MEA were determined.

 

Boron

The corresponding nominal concentrations of boron in analysed samples were 52, 91 and 162 mg boron per litre, respectively.

Analysis of the samples taken at the start of the final test showed that the actual concentrations were in agreement with nominal, 78-89% of nominal (note that the mean recoveries of the procedural recovery samples were 84-85%). The measured concentrations remained stable during the entire test period (96-101% of initial).

 

MEA

The corresponding nominal concentrations of MEA in analysed samples were 160, 280 and 500 mg MEA per litre, respectively.

Analysis of the samples taken at the start of the final test showed that the actual concentrations were in agreement with nominal, i.e 99-104% of nominal and remained stable during the entire test period (98-102% of initial).

 

Based on these results, the effect concentrations were expressed in terms of the nominal concentrations of the active ingredient.

 

The study met the acceptability criteria prescribed by the protocol and was considered valid.

 

MEA Polyborate 1:1 did not induce acute immobilisation ofDaphnia magnaat 320 mg a.i./l after 48 hours of exposure (NOEC).

 

The 48h-EC50was 423 mg a.i./l based on nominal concentrations (95% confidence interval between 320 and 560 mg a.i./l).

Description of key information

Under the conditions of a GLP OECD 202 study MEA Polyborate 1:1 did not induce acute immobilisation of Daphnia magna at 320 mg a.i./l after 48 hours of exposure (NOEC).

The 48h-EC50 was 423 mg a.i./l based on nominal concentrations (95% confidence interval between 320 and 560 mg a.i./l).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
423 mg/L

Additional information