Registration Dossier
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EC number: - | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Under the conditions of the test no biodegradation was observed after 28 days, and therefore the test material was determined to be not biodegradable., OECD 301 B, EU Method C.4-C, EPA OPPTS 835.3110, Clarke 2012.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The biodegradability of the test material was determined in a Carbon dioxide evolution test, performed under GLP conditions and in line with the standardised guidelines OECD 301 B, EU Method C.4-C and EPA OPPTS 835.3110.
Since there are difficulties associated with the evaluation of the biodegradability of organic compounds with low water solubility, a modification to the standard method of preparation of the test concentration was performed. An approach endorsed by the International Standards Organisation (ISO 1995) is to dissolve the test material in an auxiliary solvent prior to adsorption onto filter paper. High shear mixing was also applied to break up the filter paper containing the test material. Using this method the test material is evenly distributed throughout the test medium and the surface area of test material exposed to the test organisms is increased thereby increasing the potential for biodegradation.
Activated sludge was exposed to the test material for 29 days at a concentration of 5 mg carbon/L. Biodegradation was determined as CO2 evolution measured on Days 0, 2, 6, 8, 10, 14, 21, 28 ad 29.
Under the conditions of the test no biodegradation was observed after 28 days, and therefore the test material was determined to be not biodegradable.
This key study was performed in line with GLP and accepted standardised guidelines with a high standard of reporting. The study was assigned a reliability score of 1 in accordance with the criteria outlined by Klimisch (1997) and considered suitable for assessment as an accurate reflection of the test material.
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