N,N-Diethylacrylamide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 2014-02-10 to 2014-02-14

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch No.: DEAA-P
Purity: 99.95%

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L
- Sample storage conditions before analysis:kept in a refrigerator (2~8 °C), the test solution be prepared just prior to the exposure.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)

PREPARATION of test solution
-Preliminary test: prior to range-finding test, solubility test was conducted to identify the solubility of test in dilution water. As a result of solubility test, it's identified that the test substance is soluble with dilution water at the concentration of 500 mg/L.
-Range finding and limit test: the range finding and limit test are conducted at the nominal concentration of 10, 50, 80 and 100 mg/L. Each test solution of nominal concentration was prepared by dilution of stock solution in the morning of exposure date.
-Definitive test: the definitive test be conducted at the nominal concentration of 100 mg/L on the basis of range finding and limit test. Each test solution of nominal concentration was prepared by dilution of stock solution in the morning of exposure date.

TEST method: Homogeneity and stability of test substance were carried out by preliminary test according to the SOP of the test facility. The results of stability test, the concentration changes of test substance in nominal concentration (10 and 100 mg/L) after 96h were 1.1 and 12.6%, respectively. On the basis of the stability, the definitive test was carried out using static-system.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
-Common name: Medaka
- Source: Korea
- Length at study initiation (length definition, mean, range and SD): 2-4 cm
-Weight at study initiation: 0.11~0.27 g
- Method of breeding: mixed feed pellets, be fed once daily at a total rate of approximately 2%~4% of their body weight per day.

Test type:

static

Water media type:

other: Dechlorinated water

Limit test:

yes

Total exposure duration:

96 h

Hardness:

64~68 mg/L (CaCO3)

Test temperature:

22.1~23.1 °C

pH:

7.38~8.01

Dissolved oxygen:

97.3%~106%

Nominal and measured concentrations:

Nominal concentration: 100 mg/L
Measured concentration: 111.666 mg/L

Details on test conditions:

TEST SYSTEM
-temperature: 22.1 ~ 23.1°C
-light cycle: 12 hours duration
-oxygen concentration:
(1) holding on fish and acclimation period: not less than 80 per cent of air saturation value
(2) exposure period: not less than 60 per cent of air saturation value
- Test vessel: glass beaker or tank (stable from chemical and inactive).
- Type: cover for protection from evaporation of water and influx of external substance.
- No. of organisms per concentraton: 10
- Test solution volume: more than 1L/1.0 g (fish body weight)
- Control group: negative control group was composed of the dilution water

Reference substance (positive control):

yes

Remarks:

pentachlorophenol sodium

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Clinical signs and mortalities
No mortality and abnormal behavior in the negative and nominal concentration of 100 mg/L.

Body weight and total length
The range of body weight was 0.11 to 0.27g and that of total length was 2.5 to 2.9 cm in the representative test fishes.

Appearance
All the solutions were kept clear from the beginning to the end of exposure.

Validity criteria fulfilled:

yes

Conclusions:

The 48h and 96h LC50 of test item based on nominal concentration were great than 100 mg/L.
The 48h and 96h LC50 of test item based on measured concentration were great than 111.666 mg/L.

Executive summary:

The study was performed to assess the acute toxicity effect of test item in fish (Oryzias latipes). according to the OECD Guideline for Testing of Chemicals No. 203 (1992).

 

1)      The test solution was carried out using static system throughout the duration of test.

2)      There were no abnormal appearances in all the test group at the end of the test.

3)      The body weight were 0.11~0.27 g and total length were 2.5~2.9 cm in the test fish.

4)      During the test period, temperature, pH and dissolved oxygen concentration of the test solutions were 22.1~23.1°C, 7.38~8.01 and 97.3~106.0%, respectively.

5)      Hardness of the test solution were 64~68 mg/L (as CaCO3).

6)      All the test solution were kept from the beginning to the end of test.

7)      The geometric mean of measured concentration for the test substance in the nominal concentration (100 mg/L) was 111.666 mg/L.

Description of key information

The 48h and 96h LC50 of test item based on nominal concentration were great than 100 mg/L (measured concentration 111.666 mg/L).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L