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EC number: 700-556-0 | CAS number: 66682-07-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July - October 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: in accordance with guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- Principles of method if other than guideline:
- OPPTS 835.3110. Ready Biodegradability. EPA fate, transport and transformation test guidelines, 1996.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Italian Ministero della Salute
Test material
- Reference substance name:
- 1,2,3-trichloro-5-(trichloromethyl)benzene
- EC Number:
- 700-556-0
- Cas Number:
- 66682-07-3
- Molecular formula:
- C7H2Cl6
- IUPAC Name:
- 1,2,3-trichloro-5-(trichloromethyl)benzene
- Details on test material:
- - Name of test material : 3,4,5-trichloro-benzotrichloride
- Substance type: intermediate for synthesis
- Analytical purity: 97.7%
- Purity test date: 03 June 2010
- Lot/batch No.: 06/2010
- Expiration date of the lot/batch: June 2012
- Stability under test conditions: Stable away from water and heat, on iron, at temperature < 50°C. Moreover if mixed with sulphuric acid a violent hydrolysis occurs.
- Storage condition of test material: the test item is kept in glass vial. Kept away from incompatible materials (oxidisers, not noble metals, amines and alcohols). Kept away from heat and ignition sources.
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, adapted
- Details on inoculum:
- The study was performed using aerobic activated sludge from ALSI (Alto Lambro Servizi Idrici) wastewater treatment plant treating civil wastewater. The sludge was washed by centrifugation and the supernatant liquid phase decanted. A homogenized aliquot of the final sludge suspension was weighted, thereafter dried and the ratio of wet to dry weight was calculated as 8.1%.
Based on this ratio, calculated amounts of wet sludge were suspended in the test water (see next chapter) to get a concentration equivalent to 3.0 g dry material/L. During holding, the sludge was aerated at room temperature in dark conditions. Before using, the sludge inoculum was diluted with test water to obtain a final concentration of 15 mg/L (dry weight). - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Remarks:
- classic manometric method
- Details on study design:
- The assay on test item has been run in parallel with a ready biodegradable reference item, i.e. sodium benzoate, the inoculum control, the abiotic control containing the test item and a abioticizing agent (HgCl2) and the toxicity control, containing test item, reference compound and inoculum.
BOD flasks of 500 ml capacity, equipped with pressure sensors fitted on the bottle mouth, have been used as test units.
The test water was prepared according to the testing guidelines: salts of analytical grade were dissolved in purified water to obtain the following stock solutions (up to 1000 mL):
a) KH2PO4 8.50 g/L
K2HPO4 21.75 g/L
Na2HPO4 x 2H2O 33.40 g/L
NH4Cl 0.50 g/L
The pH of this solution should be 7.4
b) CaCl x 2H2O 36.40 g/L
c) MgSO4 x 7H2O 22.50 g/L
d) FeCl3 x 6 H2O 0.25 g/L
10 mL of stock solution a) and 1 mL of stock solution b), c) and d) will be combined and made up to 1000 mL with purified water. The pH of test water was 7.22, so adjustment was not needed.
Since the low water solubility of test item, the test item solutions (100 mg/L) were prepared by directly weighting into the bottles, while the reference item solution was prepared by diluting an appropriate volume of an aqueous stock solution of 10 g/L into test medium.
Activated sludge was added to each flask and the flasks were made up to a volume of 250 mL or 150 mL, according to the scale setting of the manometric device, with the test water.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Remarks:
- classic manometric method (consumption)
- Value:
- 9.4
- Sampling time:
- 28 d
- Details on results:
- The test concentration was not measured during the test, therefore all reported results are related to the nominal concentration of the test item.
The biodegradation has been followed by measuring the BOD (Biochemical Oxygen Demand) of the test item under defined conditions by the means of a manometric method.
According to OECD guideline no. 301, the oxygen consumption due to nitrification was not considered since the test substance does not contain N. The measured oxygen concentrations in the test solutions and in the inoculum control , the oxygen uptake of the reference item, abiotic control and toxicity control were used to calculate the BOD value and the % of biodegradation. ThODNH3 for 3,4,5-trichloro-benzotrichloride was calculated as 0.64 mg O2/mg and 1.67 mg O2/mg for Sodium Benzoate. the test item has not reached the 10% of biodegradation during the 28 days of test duration, having showed a maximum value of biodegradation of 9.4% at the end of the test period (mean value between two replicates). These results indicate that the test item is not ready biodegradable. The toxicity control clearly shows that the test item does not cause inhibitory effect on the microorganism activity at the tested concentration. In fact, the degradation of the reference item in presence of the test item exceeded the 25% of biodegradation at third day of exposure . In the abiotic control no biodegradation was observed, thus indicating the absence of abiotic degradation.
BOD5 / COD results
- Results with reference substance:
- The degradation of the reference item in presence of the test item exceeded the 25% of biodegradation at third day of exposure
Any other information on results incl. tables
BOD values and % biodegradation for the reference item sodium benzoate and the test item 3,4,5-trichloro-benzotrichloridein a Manometric Respirometry test.
Day of measurement |
Reference item BOD |
Reference item % biodegradation |
Test item BOD |
Test item % biodegradation |
1 | 0.20 | 12.0 | -0.02 | 0.0* |
2 | 0.41 | 24.6 | -0.02 | 0.0* |
3 | 0.63 | 37.8 | -0.02 | 0.0* |
4 | 0.76 | 45.3 | -0.03 | 0.0* |
5 | 0.97 | 57.9 | 0.01 | 1.6 |
6 | 1.09 | 65.4 | -0.05 | 0.0* |
7 | 1.18 | 70.8 | -0.05 | 0.0* |
8 | 1.21 | 72.6 | -0.05 | 0.0* |
9 | n.d. | n.d. | n.d. | n.d. |
10 | n.d. | n.d. | n.d. | n.d. |
11 | 1.29 | 77.1 | -0.04 | 0.0* |
12 | 1.31 | 78.3 | -0.06 | 0.0* |
13 | 1.41 | 84.3 | -0.06 | 9.4 |
14 | 1.37 | 82.2 | -0.02 | 2.3 |
15 | 1.38 | 82.8 | -0.15 | 0.0* |
16 | n.d. | n.d. | n.d. | n.d. |
17 | n.d. | n.d. | n.d. | n.d. |
18 | 1.47 | 87.9 | -0.02 | 0.0* |
19 | 1.53 | 91.8 | -0.02 | 0.0* |
20 | 1.54 | 92.4 | -0.02 | 0.0* |
21 | 1.53 | 91.5 | -0.13 | 0.0* |
22 | 1.55 | 92.7 | -0.16 | 0.0* |
23 | n.d. | n.d. | n.d. | n.d. |
24 | n.d. | n.d. | n.d. | n.d. |
25 | 1.59 | 95.4 | -0.03 | 0.0* |
26 | 1.59 | 95.1 | -0.05 | 0.0* |
27 | 1.60 | 95.7 | -0.06 | 0.0* |
28 | 1.61 | 96.3 | -0.08 | 0.0* |
n.d. not determined being not working days.
* this value was estimated as 0 being negative(< 0) as per calculation
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not ready biodegradable
- Conclusions:
- The test item 3,4,5-trichloro-benzotrichloride has showed to be not ready biodegradable under the conditions applied in a Manometric Respirometry test, having not reached the pass level (60% biodegradation), but showing a maximum value of degradation of 9.4% during the test period.
Moreover, the test item showed no inhibitory effects on the microorganism activity at the tested concentration, as demonstrated by the 25% of biodegradation exceeded in the toxicity control, already at the third day of test period. - Executive summary:
The ready biodegradability of the test item 3,4,5-trichloro-benzotrichloride was assessed in a 28-day biodegradation test following the “Manometric Respirometry” method according to the following guidelines:
- OECD guideline for testing of chemicals no., Ready Biodegradability, Manometric Respirometry test, 1992.
- OPPTS 835.3110. Ready Biodegradability. EPA fate, transport and transformation test guidelines, 1996.
- Council Regulation EC 440/2008 (C.4 Part V).
For this purpose, the test item was added to activated sludge collected from the aeration tank of a civil wastewater treatment plant and let to stay in contact with the sludge for 28 days under continuous stirring.
The tested concentration was 100 mg/L as test item. The biodegradation was followedby measuring the BOD (Biochemical Oxygen Demand) of the test item under defined conditions by means of a manometric method.
The test item is considered to be stable at the test conditions for a time corresponding to the exposure time (28 days). Therefore, all reported results are related to the nominal concentration of the test item.
The ThODNH3values were calculated to be equal to 1.67 mg O2/mg for reference item and 0.64 mg O2/mg for test item.
According to OECD guideline no. 301, the oxygen consumption due to nitrification was not considered since the test substance does not contain N.
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