(2S)-2-[[(2S)-1-ethoxy-1-oxopentan-2-yl]amino]propanoic acid

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2004

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Test material

Test material information

Constituent 1

Reference substance name:

(2S)-2-[[(2S)-1-ethoxy-1-oxopentan-2-yl]amino]propanoic acid

EC Number:

617-393-5

Cas Number:

82834-12-6

Molecular formula:

C10 H19 N O4

IUPAC Name:

(2S)-2-[[(2S)-1-ethoxy-1-oxopentan-2-yl]amino]propanoic acid

Test material form:

solid: crystalline

Details on test material:

White powder

Specific details on test material used for the study:

Batch 537481-017

Test solutions

Vehicle:

yes

Details on test solutions:

To prepare the stock solution, the test item was dissolved or dispersed directly in LC reconstituted water. The obtained preparation was agitated mechanically for up to 24 hours in an attempt to completely dissolve or disperse the test item and homogenize the solution. Water reconstituted (LC) was prepared using deionized water with a conductivity less than 10 PS/cm. The final solution after autoclaving has a pH of 7.5 ± 0.3.

Test organisms

Test organisms (species):

Scenedesmus sp.

Details on test organisms:

Strain No.: CCAP 276/20
Rapidly growing unicellular species commonly used in Europe for aquatic toxicity testing and recommended in OECD and EEC guidelines.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Test conditions

Test temperature:

between 23,2°C and 25,4°C

pH:

between 6.91and 9,40

Conductivity:

less than 10 PS/cm

Nominal and measured concentrations:

Nominal concentration : 0, 1, 10 & 100mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 3

Duration:

72

Dose descriptor:

EC50

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Conclusions:

The highest No Observed Effect Concentration on the growth rate (NOEC) at T72h was 10 mg/L, based on growth rate and biomass (p = 0.05).

The 0-72h EC50r was > 100 mg/L.
The 0-72h EC50b was estimated as close to 100mg/L