Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 603-006-7 | CAS number: 124728-62-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Aug 10, 2010 - Dec 17, 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: Guidelines for the testing of chemicals (HJ/T153-2004) and Guidelines for the testing of chemicals No. 207, China
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- not specified
- Principles of method if other than guideline:
- Due to the limited water solubility (below 100 mg/L) the test was conducted under Chinese regulation at 100 mg/kg soil.
- GLP compliance:
- no
- Remarks:
- no OECD GLP
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- A defined artificial soil of the following composition was used for this assay.
Sphagnum Peat 10 % (pH 5.5-6.0, no visible plant remains)
Kaolin Clay 20 % (kaolinite content preferably above 30 %)
Industrial Sand 68 % (fine sand should be dominant with more than 50 % of the particles between 50-200 μm)
CaC03 2 % (AR)
The moisture content of all constituents above was determined by drying a small sample at 105 °C and re-weighing and then blended in the correct proportions and mixed thoroughly. Deionised water was added, and the medium was thoroughly mixed. The test substance was mixed directly with artificial soil by motor stirring machine. A high concentration mixture of test substance and dry artificial soil was used as the test stock soil.
Test substance stock soil with various volumes was mixed thoroughly with artificial soil to prepare a set of test medium.
750 g (wet weight) of test medium placed into each container, with ten earthworms each. Those earthworms, that were washed with distilled water quickly before use, are placed on the test medium surface. The containers are covered with perforated plastic film to prevent the test medium from drying. - Test organisms (species):
- Eisenia fetida
- Details on test organisms:
- TEST ORGANISM
- Common name: Eisenia fetida
- Source: Jialiming Earthworm Breeding Farm in Tianjin, China
- Age at test initiation: 3 months
- Weight at test initiation: 0.29 +/- 0.04 g
ACCLIMATION
- Acclimation period: 7 days (14 days without medication)
- Acclimation conditions: same as test
- Health during acclimation (any mortality observed): no mortality, no other findings - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Test temperature:
- 20 +/- 22 °C
- pH:
- 7.52 - 7.84
- Moisture:
- 34.9 %
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1 L glass vessel, closed
- Amount of soil or substrate: 750 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: 4
- Composition of soil: Please refer to "Details on preparation and application of test substrate".
OTHER TEST CONDITIONS
- Photoperiod: 24 h/day
- Light intensity: 573 - 581 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
day 7 and 14
VEHICLE CONTROL PERFORMED: yes
TEST CONCENTRATIONS
A range finding study using test concentrations 0, 0.1, 1.0, 10 and 100 mg/kg dry weight of artificial soil using 2 replicates per concentration was performed. The duration was 7 days, temperature 20 +/- 2 °C and humidity 80 % with continuous light at 4000-6000 Lux.
- Results used to determine the conditions for the definitive study: yes - Nominal and measured concentrations:
- 100 mg/kg soil
- Reference substance (positive control):
- yes
- Remarks:
- Chloroacetamide
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LD50
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- The mortality of the blank control group on day 7 was 0 %. The mortality of the treatment group on day 7 was 2.5 %. The mortality of control group on day 14 was 2.5 % and 10 % for the treatment group.
- Results with reference substance (positive control):
- The 24 h LC50 of chloroacetamide was calculated as 3.3 * 10^-3 mg/cm^2 (validity crititeria: 1.5 to 4.5 * 10^-3 mg/cm^2).
- Reported statistics and error estimates:
- Standard statistical methods have been applied for data processing.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 7-day LD50 and the 14-day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 100 mg/kg dry soil weight.
- Executive summary:
In this study, the acute toxicity of the test substance to Eisenia fetida was determined in a 14 days artificial soil test. The study design was similar to OECD 207 and according to guidelines for the testing of chemicals (HJ/T153-2004) and guidelines for the testing of chemicals No. 207, China. Based on the results of range-finding test, one treatment group and one blank control group, which had four replicates of 10 worms per concentration each were included in a limit test. The nominal concentration of the treatment group was 100 mg/kg dry artificial soil weight. The number of mortalities were determined after 7 and 14 days of exposure. Mortality of the earthworms in blank control group was 2.5 % after 14 days, which was not more than 10 %, so the test was valid. The mortality of the treatment group on day 7 was 2.5 % and after 14 days 10 %. The 24 h LC50 of the positive control substance chloroacetamide was calculated as 3.3 * 10^-3 mg/cm^2, thus meeting the validity crititeria of 1.5 to 4.5 * 10^-3 mg/cm^2. Under conditions of the test, the test substance had no acute toxicity effects on the earthworm. The 7-day LD50 and the 14-day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 100 mg/kg dry soil weight.
Reference
Description of key information
The 7-day LD50 and the 14-day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 100 mg/kg dry soil weight.
Key value for chemical safety assessment
Additional information
In this study, the acute toxicity of the test substance to Eisenia fetida was determined in a 14 days artificial soil test. The study design was similar to OECD 207 and according to guidelines for the testing of chemicals (HJ/T153-2004) and guidelines for the testing of chemicals No. 207, China. Based on the results of range-finding test, one treatment group and one blank control group, which had four replicates of 10 worms per concentration each were included in a limit test. The nominal concentration of the treatment group was 100 mg/kg dry artificial soil weight. The number of mortalities were determined after 7 and 14 days of exposure. Mortality of the earthworms in blank control group was 2.5 % after 14 days, which was not more than 10 %, so the test was valid. The mortality of the treatment group on day 7 was 2.5 % and after 14 days 10 %. The 24 h LC50 of the positive control substance chloroacetamide was calculated as 3.3 * 10^-3 mg/cm^2, thus meeting the validity crititeria of 1.5 to 4.5 * 10^-3 mg/cm^2. Under conditions of the test, the test substance had no acute toxicity effects on the earthworm. The 7-day LD50 and the 14-day LD50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations were higher than 100 mg/kg dry soil weight.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.