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EC number: 415-070-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes
- Vehicle:
- no
- Details on test solutions:
- TEST SUBSTANCE
The test substance was soluble in water therefore a stock solution of 502.1 mg/l was prepared in Milli-Q water. A volume of 100 ml of prepared stock solution in 500 ml final volume corresponding to an actual concentration of 100 g/l being the maximum concentratio to be tested
CONTROL
A solution of 3,5-Dichlorophenolwas prepared by dissolving 128.80 mg in 10 ml 1N NaOH, diluted to approximately 30 ml with Milli-Q water and adding under stirring 1N H2SO4 to thje point of incipient precipitation. Finally the solution was diluted to one litre with Milli-Q water. The pH was 7.69. - Test organisms (species):
- activated sludge
- Details on inoculum:
- -Source: municipal sewage treatment plant: 'Waterschap de Dommel 's-Hertogenbosch, the Netherlands.
-Number of micro-organisms: determined as the amount of Mixed Liquor Suspended Solids (MLSS) per litre test medium
-Preparation: the sludge was coarsely sieved and washed with tap-water. A small amount of the sludge (250 ml) was weighed and dried at 100-105°C. From this result the amount suspended solids was calculated: the level was 4.0 g/l of sludge. The batch of sludge was used on subsequent days (maximum four days), therefore 50 ml of synthetic sewage feed was added to each litre of activated sludge at the end of each working day. The sludge was kept aerated at 20 ± 2 °C until use. Before use the pH was checked. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.5 - 7.6
- Nominal and measured concentrations:
- -test subatance: 100.4 mg/l
-control: 51.5, 33.0, 10.3, 3.3 and 1.0 mg/l - Details on test conditions:
- TEST SYSTEM
-Test vessel: all glass, 500 ml beakers and 300 ml oxygen bottles.
-Material, size, fill volume: 184 - 284 ml of Milli-Q water
-Aeration: yes, Clean, oil-free air, Airflow 0.5-1 l/min
OTHER TEST CONDITIONS
-Oxygen meter: Oxygen electrode (Tri Ox EO 200, WTW, FRG) supplied with a recorder (Kipp BD40).
-Sodium bicarbonate solution: 70 g NaHCOs dissolved per litre Milli-Q water
-Synthetic sewage feed:
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCl
0.4 g CaCl2.2H20
0.2 g MgS04 .7H2O
2.8 g K2HPO4
Dissolved in 1 l Milli-Q water, filtered and adjusted to pH 7.5 ± 0.5. The nutrient solution could be used for one week if stored at 4 °C. Before use, it shall be mixed thoroughly and the pH adjusted. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 30 min
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- No significant inhibition of the respiration rate of the sludge (<10 %) was recorded at 100.4 mg substance per litre (8.4%).
Acceptability of the test:
-The mean respiration rate of control 1 and 2 was 48.4 mg O2/l/hr. The difference between the controls is 4 %.
-The mean respiration rate of control 2 and 3 is 50.8 mg O2/l/hr. The difference between the controls is 5 %.
-The EC50 of 3,5 -Dichlorophenol was 11.37 mg/l (95 % fiducial limits: 7.79 - 16.78 mg/l).
-Since tha substance did not inhibit the respiration of active sludge at the maximum concentration to be tested (100 mg/l), no further testing was required. - Results with reference substance (positive control):
- At a concentration of the reference substance of 1.0 mg/l the respiration rate was inhibited to 10.9 %. The EC50 was 11.37 mg/l (95% fiducial limits: 7.79 - 16.78 mg/l). At 51.5 mg/l, the maximum concentration test the inhibition was 82.4%.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No significant inhibition of the respiration rate of the sludge (<10%) was recorded at 100.4 mg per litre (8.4%)
- Executive summary:
The inhibitory effect of the substance on aerobic waste-water bacteria was investigated in a respiration inhibition test, according to EEC Directive 67/548 amended November 18, 1987 (87/302), Part C: Methods for the determination of ecotoxicity, Publication No. L133, "Biodegradation: Activated sludge respiration inhibition test" (May 30, 1988). The oxygen concentration was measured after a contact time of 30 minutes with activated sludge fed with a standard amount of synthetic sewage feed. The test substance showed no significant inhibition (8.4 %) of the respiration rate at a concentration of 100 mg/l. Thus, the IC 50 (30 min) was >100 mg/l.
In conclusion, as the validity conditions were respected, the substance was found not to be toxic to waste-water bacteria.
Reference
Description of key information
IC50 (30 min) > 100 mg/l
Key value for chemical safety assessment
Additional information
The inhibitory effect of the substance on aerobic waste-water bacteria was investigated in a respiration inhibition test, according to EEC Directive 67/548 amended November 18, 1987 (87/302), Part C: Methods for the determination of ecotoxicity, Publication No. L133, "Biodegradation: Activated sludge respiration inhibition test" (May 30, 1988). The oxygen concentration was measured after a contact time of 30 minutes with activated sludge fed with a standard amount of synthetic sewage feed.
The IC 50 (30 min) was found to be >100 mg/l. As the validity conditions were respected, the substance was found not to be toxic to waste-water bacteria.
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