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EC number: 235-546-0 | CAS number: 12270-13-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
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- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Endpoint summary
- Stability
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.17 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limit
- Remarks:
- ca. 0.14 - 0.19
- Details on results:
- Test evaluations were done according to the guidelines by visual assessment of the mobility of the daphnids after 24 and 48 hours.
The EC50 was calculated by using the Probit model and by graphical determination on logarithmic probability paper.
The concentration of the test substance at the beginning and at the end of the test was analysed by the analytical Service Klybeck.
The EC0 and the EC100 after 48 h of exposure was 0.08 mg/L and 0.45 mg/L, respectively. - Results with reference substance (positive control):
- EC50 (24 h) = 0.9 ppm (range: 0.8 - 1.5 ppm)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-h EC50 of the test item to Daphnia magna under the conditions of the OECD test guideline 202 is 0.17 ppm, corresponding to ca. 0.17 mg/L.
- Executive summary:
The 48h acute toxicity of the test item to Daphnia magna was studied under static conditions. Daphnids were exposed to 0 (blank control), 0.08, 0.14, 0.25, 0.45, 0.78 ppm (measured concentration). Mortality/immobilisation were observed daily.
The 48h EC50was 0.17 ppm, corresponding to ca. 0.17 mg/L. The 48h EC0 based on mortality/immobilisation was 0.08 ppm, corresponding to ca. 0.08 mg/L.
Based on the results of this study, the test item would be classified in category Acute 1 & Chronic 1 in accordance with the classification system of the GHS.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age (e.g. 1stinstar): < 24 h
Test Type: Static
EC0: 0.08 mg/L
EC50: 0.17 mg/L 95% C.I.: 0.14 -0.19 mg/L
Endpoint(s) Effected: mortality/immobilisation
This information is used in a read-across approach in the assessment of the substance to be registered.
For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 04.10 - 06.10.1990 and 08.09 - 10.09.1990
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Results from test 1 are not conclusive based on lacking documentation
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- only mortality is recorded
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Details on test solutions:
- Dilution water parameters:
Dilution water: IR-Lake mixture
Specific conductance: 150 micromhos at 25°C - Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 50 ppm as calcium carbonate
- Test temperature:
- 20°C ± 1°C
- pH:
- 6.87 - 6.99
- Dissolved oxygen:
- 8.5 - 9.3 ppm
- Salinity:
- not applicable
- Conductivity:
- 140 - 150 micromhos at 25 °C
- Nominal and measured concentrations:
- First test: 1/ 50/ 100/ 1000 / 2500/ 5000/ 10 000 ppm
Second test: 0.2 / 0.4 / 0.6 / 1 ppm - Details on test conditions:
- Not reported
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 1.1 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Results from test 1
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 0.165 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: Results from test 1; recalculation of results based on concentration of active ingredient
- Details on results:
- - Mortality of control: 0
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no - Results with reference substance (positive control):
- n.a.
- Reported statistics and error estimates:
- no statistics reported
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 48 hr LC50 was 1.1 ppm, and 0.165 ppm for the active ingredient. No sublethal effects were recorded.
- Executive summary:
The 48 hr acute toxicity of the test item to Ceriodaphnia dubia was studied under static conditions. Daphnids were exposed to blank control and test chemical at (nominal) concentration of 1, 50, 100, 1000, 2500, 5000, 10000 ppm (test 1) and 0.2, 0.4, 0.6 and 1 ppm (test 2). Mortality was observed at test termination. The 48 hour LC50 reported in test 1 (after conducting an additional test) was 1.1 ppm, corresponding to 0.165 ppm for the active ingredient. The LC50 from test 2 was determined to be > 1 ppm (> 0.15 ppm a.i.). No sublethal effects were recorded in both test.
This study is classified as supplementary and does not satisfy the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age: 1stinstar
Test Type : Static
LC50: 1.1 ppm, and 0.165 ppm for the active ingredient (a.i.), respectively (results reported in test 1 after having conducted an additional experiment)
NOEC: 0.6 ppm, and 0.09 ppm for the a. i., respectively (results from test 2)
Endpoint(s) Effected: mortality
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26.-28.11.1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Part I, 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- No data.
- Vehicle:
- no
- Details on test solutions:
- No data.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: n.a.
- Source: daphnids were bred in the laboratories of the TF Product Ecology under standardized conditions
- Age of parental stock (mean and range, SD): < 24 h - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 136 mg/L CaCO3
Alkalinity: 0.8 mmol/L - Test temperature:
- 21 °C
- pH:
- 7.8 - 8.2
- Dissolved oxygen:
- Test media were saturated with oxygen at the beginning of the test.
- Salinity:
- no data
- Conductivity:
- no data
- Nominal and measured concentrations:
- nominal: 0.1, 0.18, 0.32, 0.58 and 1.0 ppm
measured mean concentration: 0.08, 0.14, 0.25, 0.45, 0.78 ppm - Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 250 ml beakers containing 100 ml test medium
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): n.a.
- Renewal rate of test solution (frequency/flow rate): /
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: bidistilled water
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Intervals of water quality measurement: at beginning and at end of the test
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility after 24 and 48 h
VEHICLE CONTROL PERFORMED: yes (blank control)
RANGE-FINDING STUDY
- Test concentrations: no details reported
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.17 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limit
- Remarks:
- ca. 0.14 - 0.19
- Details on results:
- Test evaluations were done according to the guidelines by visual assessment of the mobility of the daphnids after 24 and 48 hours.
The EC50 was calculated by using the Probit model and by graphical determination on logarithmic probability paper.
The concentration of the test substance at the beginning and at the end of the test was analysed by the analytical Service Klybeck.
The EC0 and the EC100 after 48 h of exposure was 0.08 mg/L and 0.45 mg/L, respectively. - Results with reference substance (positive control):
- EC50 (24 h) = 0.9 ppm (range: 0.8 - 1.5 ppm)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-h EC50 of the test item to Daphnia magna under the conditions of the OECD test guideline 202 is 0.17 ppm, corresponding to ca. 0.17 mg/L.
- Executive summary:
The 48h acute toxicity of the test item to Daphnia magna was studied under static conditions. Daphnids were exposed to 0 (blank control), 0.08, 0.14, 0.25, 0.45, 0.78 ppm (measured concentration). Mortality/immobilisation were observed daily.
The 48h EC50was 0.17 ppm, corresponding to ca. 0.17 mg/L. The 48h EC0 based on mortality/immobilisation was 0.08 ppm, corresponding to ca. 0.08 mg/L.
Based on the results of this study, the test item would be classified in category Acute 1 & Chronic 1 in accordance with the classification system of the GHS.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age (e.g. 1stinstar): < 24 h
Test Type: Static
EC0: 0.08 mg/L
EC50: 0.17 mg/L 95% C.I.: 0.14 -0.19 mg/L
Endpoint(s) Effected: mortality/immobilisation
Referenceopen allclose all
1) Analytical results of test substance measurements:
Nominal concentration [ppm] |
Concentration start [ppm] |
Concentration end [ppm] |
Mean value* [ppm] |
0.10 |
0.08 |
0.07 |
0.08 |
0.18 |
0.14 |
0.13 |
0.14 |
0.32 |
0.26 |
0.23 |
0.25 |
0.58 |
0.47 |
0.42 |
0.45 |
1.00 |
0.80 |
0.75 |
0.78 |
* used for calculation of EC50 value
2) Oxygen concentration and pH
The oxygen concentration and the pH was controlled at the beginning and at the end of the test.
Start: - Oxygen: saturated
- pH: 8.0 (highest test concentration)
- Temp.: 21° C
End:
Nominal Conc. [ppm] |
0.1 |
0.18 |
0.32 |
0.58 |
1.0 |
Blank |
Oxygen [%] |
96 |
96 |
96 |
97 |
96 |
97 |
pH |
7.9 |
7.8 |
7.9 |
7.9 |
7.9 |
7.7 |
Temp. [°C] |
21 |
21 |
21 |
21 |
21 |
21 |
3) Influence of the Test Item on the immobilisation of Daphnia magna:
Nominal concentration [ppm] |
No. of animals |
No. of responses |
Immobility [%]* |
||
24 h |
48 h |
24 h |
48 h |
||
0 (Blank) |
10 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
|||
0.08 |
10 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
|||
0.14 |
10 |
1 |
4 |
5 |
20 |
10 |
0 |
0 |
|||
0.25 |
10 |
9 |
9 |
95 |
95 |
10 |
10 |
10 |
|||
0.45 |
10 |
9 |
10 |
90 |
100 |
10 |
9 |
10 |
|||
0.78 |
10 |
10 |
10 |
100 |
100 |
10 |
10 |
10 |
* calculated for 20 animals
The first test (results see table 1) applied concentrations from 1 ppm up to 10 000 ppm, revealing 90 % death at 1ppm. As noted in handwriting on the test report, the LC50 after an additional acute testing is stated to be 1.1 ppm. However, it is not clear from the data reported how this result was obtained.
The second test (see results in table 2) was conducted with a smaller spacing factor between the concentrations and overall lower concentrations from 0.2 ppm to 1ppm. The LC50 was determined to be > 1.0 ppm.
Table 1: Mixing ratio and results of test # 9009049-02 (conducted: 1990-09-18 to 1990-09-20); Primary stock = 20,000 ppm; * secondary stock = 100 ppm; High conc. at 0 hr.: Dissolved Oxygen = 9.0 ppm, pH = 2.86.
Chamber |
Toxicant ppm |
Total volum mls |
Volume dilution mls |
Volume toxicant mls |
Volume transfer mls |
# of organisms |
Deaths at 48 hrs |
1 |
0 |
100 |
90 |
0 |
10 |
10 |
0 |
2 |
0 |
100 |
90 |
0 |
10 |
10 |
0 |
3 |
1 |
100 |
89 |
*1 |
10 |
10 |
9 |
4 |
1 |
100 |
89 |
*1 |
10 |
10 |
9 |
5 |
50 |
100 |
40 |
*50 |
10 |
10 |
10 |
6 |
50 |
100 |
40 |
*50 |
10 |
10 |
10 |
7 |
100 |
100 |
89.5 |
0.5 |
10 |
10 |
10 |
8 |
100 |
100 |
89.5 |
0.5 |
10 |
10 |
10 |
9 |
1000 |
100 |
85 |
5 |
10 |
10 |
10 |
10 |
1000 |
100 |
85 |
5 |
10 |
10 |
10 |
11 |
2500 |
100 |
77.5 |
12.5 |
10 |
10 |
10 |
12 |
2500 |
100 |
77.5 |
12.5 |
10 |
10 |
10 |
13 |
5000 |
100 |
65 |
25 |
10 |
10 |
10 |
14 |
5000 |
100 |
65 |
25 |
10 |
10 |
10 |
15 |
10000 |
100 |
40 |
50 |
10 |
10 |
10 |
16 |
10000 |
100 |
40 |
50 |
10 |
10 |
10 |
Table 2: Mixing ratio of test medium and toxicant and results of test # 9011113-02 (conducted: 1990-10-04 to 1990-10-06).
Chamber |
Toxicant ppm |
Total volum mls |
Volume dilution mls |
Volume toxicant mls |
Volume transfer mls |
# of organisms |
Deaths at 48 hrs |
1 |
0 |
100 |
90 |
0 |
10 |
10 |
0 |
2 |
0 |
100 |
90 |
0 |
10 |
10 |
0 |
3 |
0.2 |
100 |
88 |
2 |
10 |
10 |
0 |
4 |
0.2 |
100 |
88 |
2 |
10 |
10 |
0 |
5 |
0.4 |
100 |
86 |
4 |
10 |
10 |
0 |
6 |
0.4 |
100 |
86 |
4 |
10 |
10 |
0 |
7 |
0.6 |
100 |
84 |
6 |
10 |
10 |
0 |
8 |
0.6 |
100 |
84 |
6 |
10 |
10 |
0 |
9 |
1 |
100 |
80 |
10 |
10 |
10 |
3 |
10 |
1 |
100 |
80 |
10 |
10 |
10 |
5 |
1) Analytical results of test substance measurements:
Nominal concentration [ppm] |
Concentration start [ppm] |
Concentration end [ppm] |
Mean value* [ppm] |
0.10 |
0.08 |
0.07 |
0.08 |
0.18 |
0.14 |
0.13 |
0.14 |
0.32 |
0.26 |
0.23 |
0.25 |
0.58 |
0.47 |
0.42 |
0.45 |
1.00 |
0.80 |
0.75 |
0.78 |
* used for calculation of EC50 value
2) Oxygen concentration and pH
The oxygen concentration and the pH was controlled at the beginning and at the end of the test.
Start: - Oxygen: saturated
- pH: 8.0 (highest test concentration)
- Temp.: 21° C
End:
Nominal Conc. [ppm] |
0.1 |
0.18 |
0.32 |
0.58 |
1.0 |
Blank |
Oxygen [%] |
96 |
96 |
96 |
97 |
96 |
97 |
pH |
7.9 |
7.8 |
7.9 |
7.9 |
7.9 |
7.7 |
Temp. [°C] |
21 |
21 |
21 |
21 |
21 |
21 |
3) Influence of the Test Item on the immobilisation of Daphnia magna:
Nominal concentration [ppm] |
No. of animals |
No. of responses |
Immobility [%]* |
||
24 h |
48 h |
24 h |
48 h |
||
0 (Blank) |
10 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
|||
0.08 |
10 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
|||
0.14 |
10 |
1 |
4 |
5 |
20 |
10 |
0 |
0 |
|||
0.25 |
10 |
9 |
9 |
95 |
95 |
10 |
10 |
10 |
|||
0.45 |
10 |
9 |
10 |
90 |
100 |
10 |
9 |
10 |
|||
0.78 |
10 |
10 |
10 |
100 |
100 |
10 |
10 |
10 |
* calculated for 20 animals
Description of key information
The 48h acute toxicity of the test item to Daphnia magna was studied under static conditions in accordance with OECD test guideline 202 (Mätzler, 1992). Daphnids were exposed to 0 (blank control) and mean measured concentrations of 0.08, 0.14, 0.25, 0.45, 0.78 ppm. The 48h EC50 was 0.17 ppm, corresponding to ca. 0.17 mg/L.
As supporting study, one additional test on the target substance is presented. However, this test is not fulfilling the OECD TG criteria, and is only very briefly documented (Anonymous, 1990). The determined 48h LC50 for Ceriodaphnia dubia of 0.165 ppm (based on the active ingredient concentration) is in line with the findings of Mätzler (1992).
The 48h EC50 of ca. 0.17 mg/L (Mätzler, 1992), which is based on the test item containing > 90 % active ingredient, is carried forward as key value in the risk assessment.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.17 mg/L
Additional information
The key information is used in a read-across approach in the assessment of the substance to be registered.
For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.