3-aminomethyl-3,5,5-trimethylcyclohexylamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Cited as Directive 84/449/EEC, C.1 ("Acute toxicity for fish")

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

The concentration of the test item was measured 0, 24 48 and 72 hours after the test item application

Vehicle:

no

Details on test solutions:

Stock solution: 56.0005 g of the test item were dissolved in 2 L tap water

Test organisms (species):

Leuciscus idus

Details on test organisms:

- Strain: Leuciscus idus melanotus HECKEL
- Supplier: Eggers, Hohenwestedt
- Wild caught: no
- Age/size/weight/loading: 6 +/- 2 cm, 1.5 g average weight
- Feeding: TetraMin, approximately 3 % of body weight / day
- Pretreatment: single treatment with Zephirol 1:50,000 for 1 hour followed by 14 days under quarantine
- Feeding during test: no

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

12.7 degree dH (monthly mean Jan 1993)

Test temperature:

20 +/-1 °C

pH:

Control: 7.6 - 8.3
Treatments: 8.4 - 9.9

Dissolved oxygen:

8.1 - 9.3 mg/l

Nominal and measured concentrations:

Nominal: 70 / 100 / 140 / 200 / 280 mg/l
Measured: 70.3 / 101.8 / 142.3 / 202.0 / 281.0 mg/l (mean values)
- nominal concentrations were used for evaluation

Details on test conditions:

STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Concentration of vehicle/ solvent: 56.0005 g test substance in 2 l demineralized water, no additional solvent

DILUTION WATER
- Source: dechlorinated drinking water (Gelsenwasser AG)
- Aeration: continuous

TEST SYSTEM
- Exposure vessel type: 20 l aquarium
- Number of replicates, fish per replicate: 1, 10
- Photoperiod: 16 hours bright / 8 hours dark

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

140 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

110 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

70 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

LC50 after
24hs: 170.4 mg/L;
48h: 130.3 mg/L;
72h: 113.2 mg/L;
96h: 110.0 mg/L

Reported statistics and error estimates:

The EC50 was derived from a graphical presentation instead of using a suitable statistical tool (e.g. Probit analysis). Therefore, no error estimates were reported.

Sublethal observations / clinical signs:

- Concentration / response curve:

concentration in mg/ l	0	70	100	140	200	280
% mortality 96 h	0	0	30	100	100	100
% mortality 72 h	0	0	20	100	100	100
% mortality 48 h	0	0	10	60	100	100
% mortality 24 h	0	0	0	0	90	100

 

Validity criteria fulfilled:

yes

Conclusions:

The 96h-LC50 was determined as 110 mg/L indicating that the test substance does not pose a significant hazard towards fish.

Executive summary:

In a 96-h acute toxicity study, Leucisus idus were exposed to Isophoronediamine at nominal concentrations of 0 (control), 70, 100, 140, 200 and 280 mg/l under semi-static conditions. The 96-h LC50 was determined as 110 mg/L.

Test organism length: 6 +/- 2 cm
Test type: semi-static

LC50: 110 mg/L
Endpoint(s) monitored: mortality, sublethal effects

This study is classified as "reliable without restrictions".

Description of key information

Cited from SIAR for SIAM 18 (Paris, France, April 20–23, 2004)
"In a semistatic test with Leuciscus idus according to 84/449/EEC, C.1, 1984, fish were exposed for 96 hrs to concentrations of 70 - 280 mg/l 3-aminomethyl-3,5,5-trimethylcyclohexylamine. The LC50 (96 h) was determined to be 110 mg/l (Hüls AG, 1993a). A possible contribution of the basic properties of the test substance and of the resulting high pH (up to 9.6 at the LC100) to the observed effects was not discussed by the authors but cannot be excluded."

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

110 mg/L