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EC number: 217-752-2 | CAS number: 1948-33-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test was performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- Study was conducted to understand the effect of test chemical on the mortality to fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test solution was prepared by dissolving 1.8 mg, 3 mg, 4.8 mg, 7.6 mg & 12.2 mg of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 0.18 mg/L, 0.3 mg/L,0.48 mg/L, 0.76 mg/L & 1.22 mg/L, respectively
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Orange City Aquarium, Sitabuldi, Nagpur, MS (India)
- Length at study initiation (length definition, mean, range and SD): average 2.28 cm
- Weight at study initiation (mean and range, SD): average 0.115 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12-hour darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 25 mg of CaCO3
- Test temperature:
- 23.3-25.4°C
- pH:
- 6.24
- Dissolved oxygen:
- 5.94
- Nominal and measured concentrations:
- Nominal concentration 0.18 mg/L, 0.3 mg/L,0.48 mg/L, 0.76 mg/L & 1.22 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.3 - < 0.48 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Mortality and symptoms
Nominal conc. (mg/l)
Number of fish
Mortality (cumulated) after
24h
48h
72h
96h
0 (control)
8
-
-
-
-
0.18
8
-
-
-
-
0.3
8
-
-
1
1
0.48
8
-
1
7
-
0.76
8
1
7
-
-
1.22
8
8
-
-
-
Visible Symptoms: Fishes were swimming at the bottom of bowl
Nominal conc. (mg/l)
Number of fish
Symptoms after
24h
48h
72h
96h
0 (control)
8
NS8
NS8
NS8
NS8
0.18
8
D8
D8
D8
D8
0.3
8
D8
D8
D7
D7
Explanation of symptoms:
A: apathy
N: narcotic-like state
C: swimming near water surface
D: swimming at the bottom of bowl
M: Slow movements compared to control
NS: No abnormal symptoms
Number behind symbol for symptom= number of affected fish
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical to Zebra Fish Danio rerio was determine as >0.3 - <0.48 mg/L.
- Executive summary:
Study was conducted to access the effect of test chemical to fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. The nominal concentrations of the test chemical ranging from 0.18 o 2.4 mg/L was taken for the study and exposed to these concentrations for 96 hours.
A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test chemical to various nominal test concentrations, LC50 was determine to be >0.3 - <0.48 mg/L. . Based on the LC50, it can be consider that the chemical can be consider to be classified in to aquatic acute category 1 as per the CLP classification criteria.
Reference
Description of key information
Study was conducted to access the effect of test chemical to fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. The nominal concentrations of the test chemical ranging from 0.18 o 2.4 mg/L was taken for the study and exposed to these concentrations for 96 hours. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test chemical to various nominal test concentrations, LC50 was determine to be >0.3 - <0.48 mg/L. . Based on the LC50, it can be consider that the chemical can be consider to be classified in to aquatic acute category 1 as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.48 mg/L
Additional information
Summarized result for the toxicity of test chemical were studied and mention as below:
The first experimental key study was conducted to access the effect of test chemical to fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. The nominal concentrations of the test chemical ranging from 0.18 o 2.4 mg/L was taken for the study and exposed to these concentrations for 96 hours. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test chemical to various nominal test concentrations, LC50 was determine to be >0.3 - <0.48 mg/L. . Based on the LC50, it can be consider that the chemical can be consider to be classified in to aquatic acute category 1 as per the CLP classification criteria.
First study was supported by the second study from peer reviewed journal. Static toxicity tests were performed according to ASTM standard methods for fish. Test conducted under the static system for 48 hrs on Channel catfish. After the exposure of chemical with fish, effect on the mortality were observed. The LC50 (48 hr) value of test chemical to Ictalurus punctatus in 48 hours study on basis of effect of mortality was reported to be 0.37 mg/L. Thus based on the LC50 value, chemical consider to be toxic and classified as aquatic acute 1 as per the CLP classification criteria.
Similar static toxicity tests were performed according to ASTM standard methods for fish. Lepomis macrochirus exposed with chemical for 48 hrs. Fish were not fed during the experiment. In a 20-L glass jar, 15 L well water was used for the test. Mortality was measured at 1, 3, 6, 24, 48 hours. After the exposure of test chemical to Lepomis macrochirus in 48 hours study is reported to be 0.15 mg/L based on the mortality of fish. Thus chemical consider to be toxic and classified as aquatic acute 1 as per the CLP classification criteria.
Based on the above all studies from various sources, chemical consider to be toxic and classified in aquatic acute 1 category as per the CLP classification criteria.
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