Antimony nickel titanium rutile

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Rating in accordance with OECD SIDS (2002)

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

2001

Reference 2

Reference Type:

secondary source

Title:

SIDS - C.I. PIGMENT YELLOW 53

Author:

OECD

Year:

2002

Bibliographic source:

UNEP PUBLICATIONS

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Antimony nickel titanium rutile

EC Number:

701-459-6

Cas Number:

8007-18-9

Molecular formula:

Ni(x/3)Sb(2x/3)Ti(1-x)O2 0,1≤x≤0,3

IUPAC Name:

Antimony nickel titanium rutile

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: C.I. Pigment Yellow 53 was almost insoluble in water and showed a low toxicity by preliminary test. Consequently, the experiments were carried out with 1.00 mg/L at which precipitates and floating substances were not observed. However, it was likely that the test substance was not dissolved, because the presence of the test substance in the filtrate (the test solution was filtered with 0.45 µm membrane filter) was below the detection limit of ICP-MS operating condition.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna STRAUS
- Source: National Institute for Environmental Studies (JAPAN)
- Age of parental stock (mean and range, SD): < 24 h

- Feeding during test
- Food type: 0.2 mg carbon/day/individual (Chlorella vulgaris: Green Algae)

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

21 d

Test conditions

Hardness:

240 - 265 mg/L

Test temperature:

19.6 - 20.3 ºC

pH:

7.3 - 8.4 (control)

Dissolved oxygen:

7.5 - 8.7 mg/L

Nominal and measured concentrations:

Nominal Concentrations: control and 1.00 mg/L;
Stock Solution preparation and stability: 1000 mg/L stock solution was prepared for 1.00 mg/L tests, in the following way: 100 mg test substance was added in 100 mL dilution water. The test substance was not dissolved but dispersed in the stock solution.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 80 mL test solution in a 100 mL glass beaker with Teflon-lined sheet cap
- Renewal rate of test solution: every 24 h
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 (OECD Guideline 211)

- Photoperiod: 16:8 hours, light-darkness
- Light intensity: <800 lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

dispersed

Basis for effect:

reproduction

Effect concentrations 2

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

dispersed

Basis for effect:

reproduction

Effect concentrations 3

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

dispersed

Basis for effect:

reproduction

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

dispersed

Basis for effect:

mortality

Reported statistics and error estimates:

- Statistical Method:
a) Data Analysis: LC50 of parental mortality: Binominal
NOEC and LOEC: t-test
b) Method of Calculating Mean Measured Concentrations (i.e.arithmetic mean, geometric mean, etc.): Not described

Any other information on results incl. tables

- Effect: Reproduction

- Measured concentrations (as mg/L): 

----------------------------------------------------------------------------------------                    

Nominal                 Measured concentration (mg/L) on day:

----------------------------------------------------------------------------------------

Concentration         0       1        8        9       14      15

(mg/L)                  new    old    new    old     new    old

----------------------------------------------------------------------------------------

Control               <0.07  <0.07 <0.07 <0.07 <0.07 <0.07  

1.00                       0.88    0.83   0.90    0.15   0.94   0.88

----------------------------------------------------------------------------------------

 

- Effect data (reproduction):

21 days LC50 > 1.00 mg/L (95% C. I.: not available)

21 days EC50 > 1.00 mg/L (95% C. I.: not available)

21 days NOEC > 1.00 mg/L

21 days LOEC > 1.00 mg/L 

 

- Cumulative number of died parental daphnids:

-----------------------------------------------------------------------------------------

Nominal                 Cumulative number of died parental daphnids

Concentration                        (days)

(mg/L)              1  2  3  4  5  6  7  8  9 10

----------------------------------------------------------------------------------------

Control              0  0  0  0  0  0  0  0  0  0

1.00                   0  0  0  0  0  0  0  0  0  0

----------------------------------------------------------------------------------------

Nominal         Cumulative number of died parental daphnids

Concentration                    (days)

(mg/L)            11 12 13 14 15 16 17 18 19  20 21

---------------------------------------------------------------------------------------

Control             0   0   0   0   0   0    0   0   0    0   0

1.00                  0   1   1   1   1   1    1   1   1    1   1

--------------------------------------------------------------------------------------

- Time (days) of the first brood production: Mean;

Control: 8 days

1.00 mg/L: 8 days

 

- Mean cumulative number of offspring produced per adult: 21 days;

Control: 102.8

1.00 mg/L: 95.2 

 

- Calculation of toxic values:

The calculation of the toxic values is based on the nominal concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Antimony nickel titanium rutile is not chronically toxic to Daphnia magna in a test according to OECD 211 with test suspensions prepared at nominal concentrations far exceeding its water solubility, and the respective NOEC for reproduction is estimated with > 1 mg/L (nominal, dispersed).