Reaction products of 2-methylimidazole and 2,2'-[(1-methylethylidene)bis(4,1-phenyleneoxymethylene)]bisoxirane

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the O.E.C.D. test guideline 121 with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of 2-methylimidazole and 2,2'-[(1- methylethylidene)bis(4,1-phenyleneoxymethylene)]bisoxirane

EC Number:

701-436-0

Molecular formula:

(C15H16O2.C4H6N2.C3H5ClO)x

IUPAC Name:

Reaction products of 2-methylimidazole and 2,2'-[(1- methylethylidene)bis(4,1-phenyleneoxymethylene)]bisoxirane

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

As per IUCLID5 Sections 1.1. 1.2. 1.4. and 4.1.

Radiolabelling:

no

Study design

Test temperature:

Ambient (Column temperature).

HPLC method

Details on study design: HPLC method:

Approximately 0.02 g of test substance was placed in a tared 20 mL LSC vial, dissolved in 10 mL of tetrahydrofuran (THF), brought to volume and mixed well before analysis. A calibration curve using structurally related reference compounds of known Log Koc was established, using methanol or mobile phase (55:45 ratio of MeOH:H2O). The test substance and reference compounds of known Log Koc were injected into an HPLC (High Performance Liquid Chromatography) system. A calibration curve using retention time (Log t) versus Log Koc, was calculated by linear regression analysis from the injection data of the reference compounds. The calibration curve was calculated from literature values for the Log Koc of the reference compounds and the corresponding capacity factor, k, calculated as:

k = (tr-to) / to where tr is the retention time of the reference compound

From this curve, the unknown Log Koc of the test substance was estimated from its retention time by making injections of small samples of the test substance following identical column, elution, and detection parameters used to establish the calibration curve. The Log Koc of the test substance was determined by calculating the capacity factor (k) of the test substance. The HPLC operational parameters were as follows: Varian ProstarHIPLC equipted with a Varian Model 410 autosampler and a partial loop injector. The column was a Phenomenex Luna Cyano with 5 m particle size x 250 mm length x 4.6 mm inner diameter and the data was analyzed with Galaxie System software. The detector was a uv diode array. The Mobile Phase was a 55:45 ratio of Methanol: Water at a Flow rate of 0.8 mL/min. The Injection volume was 10 uL and the Detector wavelength was 210 nm The column was operated at ambient temperature and the Attenuation / Chart Speed was Autoscale. The Threshold / Peak Width was 1000 /2.

Results and discussion

Adsorption coefficient

Type:

log Koc

Value:

ca. 1.84

Remarks on result:

other: The HPLC column temperature was ambient.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test substance HPLC estimated Log Koc was 1.84 suggesting that the test substance has a low potential to adsorb to soil and sediment.

Executive summary:

The test substance, Bisphenol A, epichlorohydrin polymer, 2-methylimidazole condensate, was tested to determine the Log Koc value by an O.E.C.D. test guideline 121 HPLC method.The test substance HPLC estimated Log Koc was 1.84 suggesting that the test substance has a low potential to adsorb to soil and sediment.