Reaction mass of sodium potassium 7-amino-4-hydroxy-3,8-bis(2-{2-sulfonato-4-[2-(sulfonatooxy)ethanesulfonyl]phenyl}diazen-1-yl)naphthalene-2-sulfonate, sodium potassium 7-amino-3-{2-[4-(ethenesulfonyl)-2-sulfonatophenyl]diazen-1-yl}-4-hydroxy-8-(2-{2-sulfonato-4-[2-(sulfonatooxy)ethanesulfonyl]phenyl}diazen-1-yl)naphthalene-2-sulfonate, sodium potassium 7-amino-8-{2-[4-(ethenesulfonyl)-2-sulfonatophenyl]diazen-1-yl}-4-hydroxy-3-(2-{2-sulfonato-4-[2-(sulfonatooxy)ethanesulfonyl]phenyl}diazen-1-yl)naphthalene-2-sulfonate and sodium potassium 7-amino-3,8-bis({2-[4-(ethenesulfonyl)-2-sulfonatophenyl]diazen-1-yl})-4-hydroxynaphthalene-2-sulfonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

DOC Die - Away Test

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998-09-15 to 1998-10-13

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)

GLP compliance:

yes (incl. QA statement)

Remarks:

Swiss GLP

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: TVR 50
- Expiration date of the lot/batch: Sept 2004

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/vehicle: > 80 g/1 [in water]

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Mixture of polyvalent bacteria collected on September 14, 1998 from the aeration tank of a domestic sewage treatment plant, PRO RHENO Basel communal.

Sludge pretreatment:
The sludge was washed 4 times with tap water and the last time with test medium. An amount corresponding to 3 g dry material per liter (±10%) was mixed with test medium and then aerated until use.
pH value of the activated sludge: 7.0

Bacteria concentration:
26 mg/1 Suspended Solids (SS). The bacteria concentration was obtained by adding 10 ml of the pre-treated sludge (2.6 g/l SS) per 1000 ml test medium.

Duration of test (contact time):

28 d

Initial conc.:

37.5 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Reference substance:

other: D(+)-Glucose

Test performance:

Negative biodégradation values or values above 100 are given as 0% or 100%, respectively. The results expressed in % are given as full number and are mean values which were corrected by the blank control.

Parameter:

% degradation (DOC removal)

Value:

6

Sampling time:

28 d

Details on results:

Points of degradation plot (test substance): 2 % degradation after 1 d 6 % degradation after 3 d 5 % degradation after 7 d 6 % degradation after 10 d 6 % degradation after 14 d 6 % degradation after 21 d 6 % degradation after 28 d

Results with reference substance:

Points of degradation plot (reference substance): 51 % degradation after 1 d 95 % degradation after 3 d 96 % degradation after 7 d 98 % degradation after 10 d 98 % degradation after 14 d 99 % degradation after 21 d 99 % degradation after 28 d

Inhibition control: Biodegradation of the inhibition control after 14 days 50%

Abiotic control: Degradation in the abiotic control after 28 days 8%

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The biodegradation of FAT 40571/A was determined as 6% after 28 days of incubation and considered as not readily biodegradable.

Executive summary:

Ready Biodegradability of FAT 40571/A was carried out according to OECD 301 A and EU C.4-A Guideline using DOC Die -Away Test. Test system used was a mixture of polyvalent bacteria (activated sludge obtained from a communal wastewater treatment plant) and tested for duration of 28 days.

 

A static test at 20.0 - 22.0°C with test volume 200 ml used and continuously shaken in diffuse daylight. The test concentrations used in this test for test substance37.5 mg/1 DOC while for reference substance37.8 mg/1 DOC.Analysis was done to determine DOC concentrations.

 

Based on the study results, test substancebiodegradation after 28 days 6% while for reference substanceafter 14 days 98%.

 

In conclusion, the biodegradation of FAT 40571/A was determined as 6% after 28 days of incubation. The test substance did not reach the pass level of 70% for ready biodegradability in the DOC Die-Away Test either within the 10-day window or after 28 days of incubation. Therefore, FAT 40571/A cannot be assessed as readily biodegradable.

Reference 2

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998-09-25 to 1998-11-24

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: Commission Directive 87/302/EEC, Annex L 133, Part C, pp 99-105 (edition 1988)

GLP compliance:

yes (incl. QA statement)

Remarks:

Switzerland GLP

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: TVR 50
- Expiration date of the lot/batch: Swp 2004

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/vehicle: > 80 g/1 (in water)

Oxygen conditions:

aerobic

Remarks:

> 2 mg/l

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Activated sludge of a communal sewage treatment plant (ARA Pro Rheno Basel) collected on September 29, 1998 was washed twice with tap water one day before the test was started.

Duration of test (contact time):

28 d

Initial conc.:

151 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Reference substance:

other: Diethylene glycol

Parameter:

% degradation (DOC removal)

Value:

4

Sampling time:

28 d

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

3 h

Details on results:

Experimental values (test substance): 2 % degradation after 1 d; 1.5 % degradation after 5 d; 2.5 % degradation after 8 d; 1.5 % degradation after 16 d; 3.5 % degradation after 27 d and 3.5 % degradation after 28 d

Results with reference substance:

Experimental values for reference substance: 2 % degradation after 1 d; 29 % degradation after 5 d; 99 % degradation after 8 d; 100 % degradation after 16 d and 100 % degradation after 9 d

Negative biodégradation values or values above 100% are given as 0% or 100%, respectively. The results expressed in % are given as full number and are mean values of two test vessels running in parallel which were corrected by the blank control.

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The FAT 40571/A is not inherently biodegradable.

Executive summary:

The inherent biodegradability study with FAT 40571/A was carried out according to OECD 302 B and EU Part C guideline using Zahn-Wellens/EMPA Test). Test system used in this test was activated sludge obtained from a communal wastewater treatment plant for a test duration of 28 days. This test was a static test at 21.2 - 23.8°C with test volume of 2 litres and was continuously stirred and aerated provided with diffuse daylight.

Test concentration used was 151 mg/l DOC of the test substance to determine dissolved organic carbon (DOC) concentrations.

Based on the study results. The test substance adsorption after 3 h 0 % while biodegradation after 28 days was 4 % and that of reference substance Biodegradation after 9 days 100 %

Total elimination after 28 days 4 %.

In conclusion, the FAT 40571/A is not inherently biodegradable.

Description of key information

The test substance is not readily biodegradable in DOC die away test with 6 % biodegradation after 28 days of incubation. In addition, it is not inherently biodegradable in Zahn-Wellens/EMPA Test.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater

Additional information

The ready biodegradability was tested according to OECD 301 A and EU C.4-A Guideline using DOC Die -Away Test. Test system used was a mixture of polyvalent bacteria (activated sludge obtained from a communal wastewater treatment plant) and tested for duration of 28 days. A static test at 20.0 - 22.0°C with test volume 200 ml used and continuously shaken in diffuse daylight. The test concentration used in this test for test substance was 37.5 mg/l DOC and for the reference substance 37.8 mg/l DOC. Analysis was done to determine DOC concentrations.

The biodegradation rate of the test substance after 28 days was 6%; for the reference substance after 14 days 98%.

As the test substance did not reach the pass level of 70% for ready biodegradability in the DOC Die-Away Test either within the 10-day window or after 28 days of incubation, it cannot be assessed as readily biodegradable.

Under inherent biodegradability test conditions, the total elimination (regarding adsorption and biodegradation) of the substance after 28 days was 4 %. The substance is hence not inherently biodegradable.