Registration Dossier
Registration Dossier
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EC number: - | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Not readily biodegradable (13 % degradation after 28 days); OECD 301 D, EU Method C.6; Douglas (1993)
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
If orthoboric acid, potassium salt could be isolated from the chemical matrix in which it is made, it would not be tested in a ready biodegradation test because it is inorganic.
However, the ready biodegradability of orthoboric acid, potassium salt as manufactured (45% w/w in highly refined mineral oil) was determined in accordance with the standardised guidelines OCED 301 D and EU Method C.6 by the measurement of dissolved oxygen, and Chemical Oxygen Demand (COD), in a closed bottle test. During the study, sealed bottles containing the test material (adsorbed onto glass filter paper) and inorganic nutrient medium were inoculated with activated sewage sludge bacteria and incubated for up to 28 days at 20 ± 1 ºC. On days 0, 5, 15 and 28 dissolved oxygen measurements were performed electrochemically. Percentage biodegradation values were determined by comparing the extent of oxygen depletion with the Theoretical Oxygen Demand (2.109 mg O2/mg). The Chemical Oxygen Demand (COD) of the test material was determined, and a slightly higher value was obtained (2.84 mg O2/mg). Additional bottles, containing both the test material and a reference substance were prepared in order to provide additional information on the inhibitory effect of the test material. Under the conditions of the study, the test material attained only 13 % biodegradation after 28 days and cannot, therefore, be termed as readily biodegradable. The test material was not found to be inhibitory to activated sewage sludge bacteria under the conditions of the study. The study was performed in line with GLP and accepted standardised guidelines with a high standard of reporting and assigned a reliability score of 1.
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