5-ethylidene-8,9,10-trinorborn-2-ene, manufacture of, by-products from

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

5th June - 3rd October 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

Version / remarks:

paragraph q.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3a,4,7,7a-tetrahydro-1H-indene

EC Number:

221-260-3

EC Name:

3a,4,7,7a-tetrahydro-1H-indene

Cas Number:

3048-65-5

Molecular formula:

C9H12

IUPAC Name:

3a,4,7,7a-tetrahydro-1H-indene

Constituent 2

Reference substance name:

4-vinylcyclohexene

EC Number:

202-848-9

EC Name:

4-vinylcyclohexene

Cas Number:

100-40-3

Molecular formula:

C8H12

IUPAC Name:

4-vinylcyclohexene

Constituent 3

Reference substance name:

Cyclopentadiene

EC Number:

208-835-4

EC Name:

Cyclopentadiene

Cas Number:

542-92-7

Molecular formula:

C5H6

IUPAC Name:

cyclopenta-1,3-diene

Constituent 4

Reference substance name:

cis-1,2-Divinylcyclobutane

Cas Number:

2422-85-7

Molecular formula:

C8H12

IUPAC Name:

cis-1,2-Divinylcyclobutane

Constituent 5

Reference substance name:

Ethylbenzene

EC Number:

202-849-4

EC Name:

Ethylbenzene

Cas Number:

100-41-4

Molecular formula:

C8H10

IUPAC Name:

ethylbenzene

Constituent 6

Reference substance name:

5-vinylnorborn-2-ene

EC Number:

221-259-8

EC Name:

5-vinylnorborn-2-ene

Cas Number:

3048-64-4

Molecular formula:

C9H12

IUPAC Name:

5-vinylbicyclo[2.2.1]hept-2-ene

Constituent 7

Reference substance name:

Cycloocta-1,5-diene

EC Number:

203-907-1

EC Name:

Cycloocta-1,5-diene

Cas Number:

111-78-4

Molecular formula:

C8H12

IUPAC Name:

cycloocta-1,5-diene

Constituent 8

Reference substance name:

3a,4,7,7a-tetrahydro-4,7-methanoindene

EC Number:

201-052-9

EC Name:

3a,4,7,7a-tetrahydro-4,7-methanoindene

Cas Number:

77-73-6

Molecular formula:

C10H12

IUPAC Name:

3a,4,7,7a-tetrahydro-4,7-methanoindene,

Constituent 9

Reference substance name:

3a,4,7,7a-tetrahydro-4-methyl-1H-indene

EC Number:

231-021-5

EC Name:

3a,4,7,7a-tetrahydro-4-methyl-1H-indene

Cas Number:

7413-02-7

Molecular formula:

C10H14

IUPAC Name:

4-methyl-3a,4,7,7a-tetrahydro-1H-indene

impurity 1

Reference substance name:

Combination of: 6-(cyclohex-3-en-1-yl)bicyclo[2.2.1]hept-2-ene 2-vinyl-1,2,3,4,4a,5,8,8a-octahydro-1,4-methanonaphthalene 6-vinyl-1,4,4a,5,6,7,8,8a-octahydro-1,4-methanonaphthalene 2-vinyl-1,2,3,4,4a,5,8,8a-octahydro-1,4:5,8-dimethanonaphthalene 2,2'-bi(bicyclo[2.2.1]heptane-5,5'-diene) 3a,4,4a,5,8,8a,9,9a-octahydro-1H-5,8-methanocyclopenta[b]naphthalene 4,4a,4b,5,8,8a,9,9a-octahydro-1H-1,4-methanofluorene

Molecular formula:

C13H18 to C14H18

IUPAC Name:

Combination of: 6-(cyclohex-3-en-1-yl)bicyclo[2.2.1]hept-2-ene 2-vinyl-1,2,3,4,4a,5,8,8a-octahydro-1,4-methanonaphthalene 6-vinyl-1,4,4a,5,6,7,8,8a-octahydro-1,4-methanonaphthalene 2-vinyl-1,2,3,4,4a,5,8,8a-octahydro-1,4:5,8-dimethanonaphthalene 2,2'-bi(bicyclo[2.2.1]heptane-5,5'-diene) 3a,4,4a,5,8,8a,9,9a-octahydro-1H-5,8-methanocyclopenta[b]naphthalene 4,4a,4b,5,8,8a,9,9a-octahydro-1H-1,4-methanofluorene

Constituent 10

Reference substance name:

4,4a,4b,5,8,8a,9,9a-octahydro-1H-1,4-methanofluorene OR 4,4a,4b,5,8,8a,9,9a-octahydro-1H-1,4:5,8-dimethanofluorene

Molecular formula:

C14H18 to C15H18

IUPAC Name:

4,4a,4b,5,8,8a,9,9a-octahydro-1H-1,4-methanofluorene OR 4,4a,4b,5,8,8a,9,9a-octahydro-1H-1,4:5,8-dimethanofluorene

Constituent 11

Reference substance name:

3a,4,4a,5,8,8a,9,9a-octahydro-4,9:5,8-dimethano-1H-benz[f]indene

EC Number:

230-512-1

EC Name:

3a,4,4a,5,8,8a,9,9a-octahydro-4,9:5,8-dimethano-1H-benz[f]indene

Cas Number:

7158-25-0

Molecular formula:

C15H18

IUPAC Name:

3a,4,4a,5,8,8a,9,9a-octahydro-1H-4,9:5,8-dimethanocyclopenta[b]naphthalene

Constituent 12

Reference substance name:

Tetramers of cyclopentadiene and 1,3-butadiene

Molecular formula:

C16H24 to C20H24

IUPAC Name:

Tetramers of cyclopentadiene and 1,3-butadiene

Test material form:

liquid

Details on test material:

Light brown liquid.
Batch number 776809

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Severn Trent WWTP Loughborough, Leicestershire on 5/8/19.
- Water filtered: yes, first 200mL discarded,
- Type and size of filter used, if any: coarse
- maintained on aeration at 21-22C prior to use

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium as used: KH2PO4 85mg/L, K2HPO4 217.5mg/L, Na2HPO4.2H2O 334mg/L, NH4Cl 5mg/L, CaCL2 27.5mg/L, MgSO4.7H20 22.5mg/L, FeCL3.6H20 0.25mg/L. Adjusted from pH 7.7 to 7.4 using HCl.
- Solubilising agent (type and concentration if used):none
- Test temperature: 20-22C
- pH: 7.4
- pH adjusted: / no
- CEC (meq/100 g):
- Aeration of dilution water: no
- Suspended solids concentration:
- Continuous darkness: no
- Replicates: Test performed in duplicate

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: using substrate and sodium benzoate

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

2

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Sodium benzoate showed 92% degradation after 14 days with more than 60% degradation in 10 days window. 97% degradation after 28 days.

Any other information on results incl. tables

The mean BOD of the innoculated mineral medium control was 15.6mg O2/L after 28 days and therefore satisfied the validation criteria of the test guidelines. The pH of the innoculated test items was 7.5 at day 28 and therefore within permissible limits. The difference in the results between the two replicates was within the permissible 20% variation. The toxicity control attained 30% biodegradation after 14 days and 32% after 28 days confirming that the test item was not toxic to the sewage treatment microorganism used in the study.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Executive summary:

In a guideline and GLP manometric respiration ready biodegradation study, the substance THI TM was only degraded by 2%. It is not therefore readily biodegradable.