Reaction products of 4-Diazo-3,4-dihydro-7-nitro-3-oxo-1-naphthalenesulfonic acid coupled with 7-Amino-4-hydroxy-2-naphthalenesulfonic acid, subsequently chelated with Chromium(3+) and cobalt(3+), further mixed with reaction products of 2,4,6-Trichloro-1,3,5-triazine and Ethanol, 2-[(4-aminophenyl)sulfonyl]-, 1-(hydrogen sulfate), sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From January 12, 2018 to March 01, 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: Daphnia magna was obtained from the CAROLINA BIOLOGICAL SUPPLY COMPANY - Burlington, North Carolina (USA) and maintained as a stock culture at BIOAGRI.

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test temperature:

20.1 to 20.3°C

pH:

7.7 to 8.3

Dissolved oxygen:

7.8 to 8.1 mg/L

Nominal and measured concentrations:

Nominal concentrations for definitive test: 0.65; 1.3; 2.5; 5 and 10 mg/L.

Details on test conditions:

- Test volume: 15 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 replicates for per test concentration a
- No. of vessels per control (replicates): 4
- Photoperiod: 16 hours of light and 8 hours of dark

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.95 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

The 24h-EC50 of reference substance (potassium dichromate) was 1.05 mg/l.

Table 1. Number of Immobile Organisms at 24 and 48 Hours of Exposure, During the Definitive Test.

Test concentration (nominal) (mg/L)	Number of immobile organisms
	24 hours					48 hours
	a	b	c	d	% of total immobility	a	b	c	d	% of total immobility
10	5	5	5	5	100	5	5	5	5	100
5	3	2	2	2	45	4	2	3	2	55
2.5	1	1	0	1	15	1	2	2	1	30
1.3	0	0	0	0	0	0	0	0	0	0
0.65	0	0	0	0	0	0	0	0	0	0
Control	0	0	0	0	0	0	0	0	0	0

a, b, c and d = replicates with 5 organisms each.

 

Table 2. Number of Immobile Organisms at 24 Hours of Exposure, During the Test with the Reference Substance.

Concentration (mg/L)	Number of immobile organisms
	24 hours
	a	b	c	d	% of total immobility
2.4	5	5	5	5	100
1.8	5	4	5	4	90
1.0	3	3	2	2	50
0.56	0	0	0	0	0
0.32	0	0	0	0	0
Control	0	0	0	0	0

a, b, c and d = replicates with 5 organisms each.

 

Table 3. Dissolved Oxygen and pH of the Tested Concentrations and Control, at the Beginning and at the End of the Definitive Test.

Nominal concentrations (mg/L)	pH		Dissolved oxygen (mg/L)
	0 hour	48 hours	0 hour	48 hours
10	8.2	8.3	8.1	7.9
5	8.1	8.2	8.0	7.9
2.5	7.9	8.0	7.9	7.7
1.3	7.9	8.0	8.0	7.8
0.65	7.8	8.0	8.0	7.8
Control	7.7	7.9	8.1	7.9

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 202 test method, the 48-hr EC50 of Everzol SR98 was 3.95 mg/L.

Executive summary:

This test using the procedures outlined in the BIOAGRI Study Plan for 16264.206.071.17 and OECD 202 (OECD, 2004). The results of this OECD 202 test for Everzol SR98 show that all validity criteria were met. Under the conditions of this test, the 48-hr EC50 of Everzol SR98 was 3.95 mg/L.

Description of key information

The 48-hr EC₅₀ of Everzol SR98 was 3.95 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3.95 mg/L

Additional information

Not more than 10 % of the Daphnia were immobilised in the control and the dissolved oxygen concentration at the end of the test was ≥3 mg O₂/L. All test validity criteria were met. The values of pH of the control and test solutions measured at 0 and 48 hours not deviate more than 1.5 units during the test. The 24-hours EC50 value of the reference substance (potassium dichromate) was 1.05 mg/L, showing the sensitivity of the test system. Under the conditions of this test, the 48h-EC₅₀ of Everzol SR98 was 3.95 mg/L.