Gum of Cistus ladaniferus (Cistaceae) obtained from stems and leaves by extraction with alkaline solution

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 22 to July 03, 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The substance is considered to be adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Version / remarks:

adopted 02. Oct. 2012

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Version / remarks:

Council Regulation (EC) No 440/2008 of 30 May 2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

inspected on 29 and 30 November 2010 / signed on 11 April 2011

Type of method:

pycnometer method

Key result

Type:

density

Density:

1.116 g/cm³

Temp.:

20 °C

Key result

Type:

relative density

Density:

1.116 other: unitless

Temp.:

20 °C

Table 4.4/1: Measured Values

First Determination				Error rel.	Error abs.
Temperature	19.8	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liq.	Pycn. + test item	Pycn. + test item + repl. liq.
Mass in g	36.9435	61.8151	61.8159	39.0239	62.0338
Temperature in °C	20.4	19.8	20.0	20.2	20.0
Volume of Pycnometer	24.9164	cm^3
Density of Replacement Liquid	0.9982			Error rel.	Error abs.
Density of Test Item	1.1150	g/cm^3		0.0981%	0.0011
Second Determination				Error rel.	Error abs.
Temperature	20.2	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liq.	Pycn. + test item	Pycn. + test item + repl. liq.
Mass in g	36.9434	61.8141	61.8160	39.1070	62.0452
Temperature in °C	20.0	20.2	19.8	20.2	19.8
Volume of Pycnometer	24.9155	cm^3
Density of Replacement Liquid	0.9983			Error rel.	Error abs.
Density of Test Item	1.1166	g/cm^3		0.0946%	0.0011

Results of the single determinations with the respective error as well as the mean value and its standard deviation are presented in the following tables:

Table 4.4/2: Results of Single Determinations

 

Determination	Density in g/cm^3	Error of Determ. in g/cm^3
1	1.1150	0.0011
2	1.1166	0.0011

 

Table 4.4/3 Results – Mean Values

 

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	24.92	cm^3	0.0006	0.00%
Density of Test Item	1.1158	g/ cm^3	0.0011	0.10%
Density of Test Item	1115.8	Kg/m^3	1.1	0.10%
Relative Density of Test Item	1.1158		0.0011	0.10%

 

The density of the test item was calculated as 1.1158 ± 0.0011 g/cm³ at 20.0 ± 0.4 °C, which is the mean of two independent determinations ± the standard deviation. Standard deviation shows good correlation of the two determinations. Therefore, the study is considered valid.

Conclusions:

The density of the test substance was calculated as 1.1158 ± 0.0011 g/cm3 at 20.0 ± 0.4 °C and the relative density is determined as D20/4 = 1.1158 ± 0.0004
Denser than water

Executive summary:

The density of the test substance was measured under GLP according OECD 109 / EU A3 guideline, using the pycnometer method.

Two determinations were performed and the mean value was retained.

This result was then converted to relative density towards water at4°C.

Mean density: 1.1158± 0.0011 g/cm³at 20.0 ± 0.4 °C

Mean relative density (D20/4) : 1.1158

Description of key information

The density of the test item was calculated as 1.1158 ± 0.0011 g/cm3 at 20.0 ± 0.4 °C and the relative density is determined as D20/4 = 1.1158

Key value for chemical safety assessment

Relative density at 20C:

1.116

Additional information

A fully reliable experimental study, conducted under GLP according to OECD 109 /EU A3 guideline, using pycnometer method, is available. It is considered as a key study, and the result is retained as key data.