Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2018-09-25 to 2018-10-18
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
No samples were used from the test. The test substance was analytical verified via a separate analytic in Daphnia medium at concentrations of 2, 20, 30 and 40 mg/L.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls and Treatment: 5 daphnids per replicate, 4 replicates in total
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna straus, Crustacean, Cladocero
- Source: Eurofins Biolab Test Facility
- Age of parental stock (mean and range, SD): 6-24 hours (Breeder)
- Feeding during test: No


ACCLIMATION
- Acclimation conditions (same as test or not): Same as test
- Type of food: Green alga, Pseudokirchneriella subcapitata and Saccharomices cerevisiae
- Health during acclimation (any mortality observed): No
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
140 - 250 mg CaCO3/L
Test temperature:
20 - 20.2°C
pH:
Control: 8.04 - 7.78
Treatment: 8.01 - 7.77
Dissolved oxygen:
Control: 5.44 - 6.20 mg/L
Treatment: 5.77 - 6.02 mg/L
Nominal and measured concentrations:
Nominal: 0 and 100 mg/L
Measured: 0 and 106 mg/L (0h); 0 and 102 mg/L (48h)
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beaker
- Type (delete if not applicable): open
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: No.
- Photoperiod: None in the course of the test.
- Light intensity: No lightning in the course of the test.

RANGE-FINDING STUDY
- Test concentrations: Ten-fold dilutions from 1000 mg/L to 0.1 mg/L.
- Results used to determine the conditions for the definitive study: Yes
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Table 1: Number of immobilised Daphnia at 24 and 48 hours in the control and in the treated groups (test item)

 

Control

24 hours

48 hours

Control replication N.1

0/5

0/5

Control replication N.2

0/5

0/5

Control replication N.3

0/5

0/5

Control replication N.4

0/5

0/5

 

Concentration 1: 100 mg/L

24 hours

48 hours

Treated -replication N.1

0/5

0/5

Treated -replication N.2

0/5

0/5

Treated -replication N.3

0/5

0/5

Treated -replication N.4

0/5

0/5

 

Validity criteria fulfilled:
yes
Conclusions:
The toxicity of 3-methyl-1,3-butandiol acetate was determined in an OECD 202 study with Daphnia magna and in compliance with GLP. Daphnids were exposed to the test substance in a concentration of 100 mg/L for 48h. Test concentration was analytically verified via GC-FID. No immobility of Daphnids were observed after 24 and 48h. The EC50 value after 24 and 48h was determined with > 100 mg/L based on nominal concentration.

Description of key information

EC50 (24h) = > 100 mg/L

EC50 (48h) = > 100 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information

The toxicity of 3-methyl-1,3-butandiol acetate was determined in an OECD 202 study with Daphnia magna and in compliance with GLP. Daphnids were exposed to the test substance in a concentration of 100 mg/L for 48h. Test concentration was analytically verified via GC-FID. No immobility of Daphnids were observed after 24 and 48h. The EC50 value after 24 and 48h was determined with > 100 mg/L based on nominal concentration.