Cyclopropanecarboxylic acid, [(3S,4R,4aR,6S,6aS,12R,12aS,12bS)-3-[(cyclopropylcarbonyl)oxy]-1,3,4,4a,5,6,6a,12,12a,12b-decahydro-6,12-dihydroxy-4,6a,12b-trimethyl-11-oxo-9-(3-pyridinyl)-2H,11H-naphtho[2,1-b]pyrano[3,4-e]pyran-4-yl]methyl ester

Short-term toxicity to fish

Description of key information

Freshwater: 

In a key 96 hours semi-static acute toxicity study test (Eco-Science Corporation, SG-ES2011-B05C, 2011) with Cyprinus carpio the LC50 for the test substance was determined to be 18 mg/L based on the nominal concentrations.

Marine Water:

In a 96 hours static acute toxicity key study (Wildlife International, 394396, 2012)

with juvenile sheepshead minnow the LC50 (96 h) for the test substance was determined to be > 31 mg/L, the highest concentration tested, based on the mean measured concentrations. The 96-hour NOEC was 31 mg/L, based on mean measured concentration.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

18 mg/L

Marine water fish

Marine water fish

Effect concentration:

31 mg/L

Additional information

 

Freshwater:

The acute toxicity of the test substance to Cyprinus carpio was determined in a 96-hour semi-static key test (Eco-Science Corporation, SG-ES2011-B05C, 2011) according to the guideline JMAFF (12-Nousan-8147, November 24, 2000) guideline equivalent to the OECD 203 (1992). The nominal concentrations tested were 4.9, 7.4, 11, 17 and 25 mg/L. The study included also a negative control and a solvent control group. The fish were tested in groups of 10 animals in 60-L glas aquaria containing 50 L test solution. Fish were observed for survival and symptoms of toxicity at 24, 48, 72 and 96 hours after starting of exposure. The mean measured concentration of the test item ranged from 90.9 % to 100.0 % of nominal concentrations. The biological results are based on nominal concentrations. Stock solutions were prepared by solving the appropriate amounts of the test item in Dimethylformamide. Aliquots of the stock solutions were diluted with freshwater to achieve the test concentrations. The LC50 for the test substance was determined to be 18 mg/L based on the nominal concentrations.

 

In addition a supporting study (BASF SE, 394395, 2015) was performed under static conditions with fathead minnow (Pimephales promelas). The LC50 after 96 hours was determined to be 18.3 mg/L based on mean measured concentrations.

 

Furthermore a supporting study (BASF SE, 732014, 2015) was performed with Rainbow Trout (Oncorhynchus mykiss). In this case the 96h-LC50 was determined to be greater than 21.3 mg/L.

 

Marine water:

In a static acute toxicity laboratory study (Wildlife International, 394396, 2012), juvenile sheepshead minnows (Cyprinodon variegatus) were exposed to nominal concentrations of 9.8, 14.5, 21, 31 and 46 mg/L, corresponding to the mean measured concentrations 10.5, 14.7, 21, 26 and 31 mg/L, respectively, in groups of 10 animals in stainless steel aquaria containing 15 L filtered seawater (salinity: 20 ‰) with 2 replicates per concentration. Fish were observed for survival and symptoms of toxicity within 3.5, 24, 48, 72 and 96 hours after starting of exposure. The biological results are based on the mean measured concentrations. After 96 hours of exposure all fish in the control group appeared normal throughout the test. There was one mortality among fish in the 10.5 mg/L treatment group within 24 hours of test initiation. The single mortality in the 10.5 mg/L treatment group was isolated in nature and no signs of toxicity or mortality were observed at the higher test concentrations; therefore, this mortality was not considered to be treatment related. All remaining sheepshead minnows in all treatment groups appeared normal throughout the test, with no mortalities or overt signs of toxicity observed.  The LC50 (96 h) for the test substance was determined to be > 31 mg/L, the highest concentration tested, based on the mean measured concentrations.