Reaction mass ofDisodium [1-{[2-(hydroxy-kO)-3,5-dinitrophenyl]diazenyl-kN1}naphthalen-2-olato(2-)-kO][3-(hydroxy-kO)-4-{[2-(hydroxy-kO)naphthalen-1-yl]diazenyl-kN1}-7-nitronaphthalene-1-sulfonato(3-)]chromate(2-) andDisodium [1-{[2-(hydroxy-kO)-3,5-dinitrophenyl]diazenyl-kN2}naphthalen-2-olato(2-)-kO][3-(hydroxy-kO)-4-{[2-(hydroxy-kO)naphthalen-1-yl]diazenyl-kN1}-7-nitronaphthalene-1-sulfonato(3-)]chromate(2-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental starting date - 05 October 1993; Experimental termination date - 07 October 1993; Study completion date - 24 January 1994.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Code number for the test material as used in the study report: FAT 20037/C
- Source and batch No.of test material: 9300001
- Expiration date of the batch: February 1998

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature

OTHER SPECIFICS:
- Purity: ca. 80 %
- Appearance: solid
- Solubility: >2 g/L (in water)

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: the testing facility of the Product Ecology TF, Textile Dyes Division, CIBA-GEIGY Ltd., Basle
- Age at study initiation: young daphnids
- Method of breeding: Cultures of Daphnia magna are maintained in glass vessels containing aprox. 3.0 L of reconstituted water at 18 - 22 °C. The water was renewed partially three times weekly. The daphnia were feed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantités that the food was consumed within 24 h.
Pretreatment: 24 hours before the start of the exposure reproductive daphnia were separated from the young daphnia by sieving all individuals through a 710 µrn sieve.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

136 mg/L CaCO3

Test temperature:

21.5 °C

pH:

7.8 - 8.2

Dissolved oxygen:

96-99 %

Nominal and measured concentrations:

58, 100 and 180 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: Beaker
- Material, size, headspace, fill volume: 250 mL Glass beaker.
- Volume of solution: 100-200 mL test medium.
- No. of organisms per vessel: 10
- No. of vessels per concentration: Duplicate
- No. of vessels per control: Duplicate

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Bi-distilled water.
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1
- Culture medium different from test medium: No


OTHER TEST CONDITIONS
- Photoperiod: 16 hours
- pH: 7.8 - 8.2


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobilization.

VEHICLE CONTROL PERFORMED: yes:

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 10 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

Potassium dichromate (EC50: 0.96 mg/L)

The reported results were related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 4.8 - 5.6 % of the nominal values. 2.9, 5.5 and 10.0 mg/L were the measured concentrations for nominal concentrations of 58, 100 and 180 mg/L, respectively. The test concentrations were prepared from the supernatant of a 1 g/L stock solution, in which most of the substance was settled down. For the preparation of the test concentrations only the supernatant of the stock solution was taken. In all test concentrations non alteration of the test substance after 48 hours was observed. In the control and in all test concentrations, no immobilization of the daphnia was observed after 48 hours. The EC0 is at least 10.0 mg/L, but might even be higher than this concentration. The EC50 and EC100 values are clearly higher than 10.0 mg/L. Therefore, the EC50 value could not be calculated.

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 of the test substance towards aquatic invertebrates >10 mg/L.

Executive summary:

The short-term toxicity potential of the test item to aquatic invertebrates was investigated in a study conducted according to OECD Guideline 202 and EU Method C.2, in compliance with GLP. Based on a pretest, the test concentrations were chosen as 58, 100 and 180 mg/L nominal. For each concentration, of test substance, 20 daphnids divided in two groups of 10 animals were used. The daphnids were exposed to the test article in 250 mL beakers containing 100 - 200 mL test medium with 10 animals per beaker. All tests were run in duplicate. During exposure the animals were kept at 21 ± 1 °C with 16 hours illumination. Test evaluations were done according to the guidelines by visual assessment of mobility of the daphnia after 24 and 48 hours. During test duration the test article concentrations in the analysed test media were in the range of 4.8 - 5.6 % of the nominal values. 2.9, 5.5 and 10.0 mg/L were the measured concentrations for nominal concentrations of 58, 100 and 180 mg/L, respectively. Hence, the reported results were related to the analytical concentrations of the test article. In all test concentrations no alteration of the test substance after 48 hours was observed. In the control and in all test concentrations, no immobilization of the daphnids was observed after 48 hours. The EC0 is at least 10 mg/L, but might even be higher than this concentration. The EC50 and EC100 values are clearly higher than 10 mg/L. Therefore, the EC50 value could not be calculated.

Description of key information

The short-term toxicity potential of the test item to aquatic invertebrates was investigated in a study conducted according to OECD Guideline 202 and EU Method C.2, in compliance with GLP.Based on a pre-test, the test concentrations were chosen as 58, 100 and 180 mg/L nominal. For each concentration, of test substance, 20 daphnids divided in two groups of 10 animals were used. The daphnids were exposed to the test article in 250 mL beakers containing 100 - 200 mL test medium with 10 animals per beaker. All tests were run in duplicate. During exposure the animals were kept at 21 ± 1°C with 16 hours illumination. Test evaluations were done according to the guidelines by visual assessment of mobility of the daphnia after 24 and 48 hours. During test duration the test article concentrations in the analysed test media were in the range of 4.8 - 5.6 % of the nominal values. 2.9, 5.5 and 10.0 mg/L were the measured concentrations for nominal concentrations of 58, 100 and 180 mg/L, respectively. Hence, the reported results were related to the analytical concentrations of the test article. In all test concentrations no alteration of the test substance after 48 hours was observed. In the control and in all test concentrations, no immobilization of the daphnids was observed after 48 hours. The EC0 is at least 10 mg/L, but might even be higher than this concentration. The EC50 and EC100 values are clearly higher than 10 mg/L. Therefore, the EC50 value could not be calculated.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

10 mg/L