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Diss Factsheets
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EC number: 943-533-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of 6-ethyl-3-methyloct-5-en-1-ol and 6-ethyl-3-methyloct-6-en-1-ol
- Molecular formula:
- C11H22O
- IUPAC Name:
- Reaction mass of 6-ethyl-3-methyloct-5-en-1-ol and 6-ethyl-3-methyloct-6-en-1-ol
- Test material form:
- other: liquid
- Details on test material:
- Test substance name: Super Muguet
Chemical name: 6-ethyl-3-methyl-6(5)-octen-1-ol
CAS number: 266330-64-3 & 26330-65-4
Molecular weight: 170.29
Batch number: SC00011582
Purity: 98.3 % (sum of isomers)
Storage: Room temperature (15 - 30 °C)
Expiry date: 20 June 2016
Constituent 1
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Nominal test concentrations:
Range finding test: 0.06, 0.6, 6.0 and 60 mg/L
Definitive test: 0.572, 1.831, 5.859, 18.75 and 60 mg/L
Geometric mean measured concentrations:
Definitive test: 0.389, 1.118, 3.768, 13.648 and 44.002 mg/L
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test organism, Psuedokirchnerella subcapitata (Strain 278/4), was originally obtained from the Culture Collection of Algae and Protozoa (CCAP) and is a representative species of the freshwater aquatic phytoplankton. This species is recommended for testing in accordance with the OECD regulatory guidelines.
Prior to testing, duplicate starter cultures were prepared and incubated under test conditions to obtain sufficient algal cells in exponential growth and to achieve a starting algae density of 1 x 10^4 cells/mL.
Positive control tests are conducted periodically to assess the performance of the species.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- Temperature incubation: 22.3 - 23.3 °C
- pH:
- pH increase during the test: 7.60 - 9.30. This increase of more than 1.5 units was thought not to have impacted the test as the control validility criterea were achieved.
- Salinity:
- no (freshwater)
- Nominal and measured concentrations:
- Nominal test concentrations:
Range finding test: 0.06, 0.6, 6.0 and 60 mg/L
Definitive test: 0.572, 1.831, 5.859, 18.75 and 60 mg/L
Geometric mean measured concentrations:
Definitive test: 0.389, 1.118, 3.768, 13.648 and 44.002 mg/L - Details on test conditions:
- Light Intensity: 7300 Lux
Orbital Shaker Speed: 100 rpm
Test vessel: Sterlie autoclaved glass 250 mL Erlenmeyer (conical) flask, into which 100 mL of the appropriate control or test media was added - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.938 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 3.074 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.289 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.118 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Effects based on yield and biomass were also reported (see attached full study report). However, the preferred observational endpoint in the algal inhibition study is growth rate because it is not dependent on the test design (ECHA guidance Chapter R.7b v1.1). The EU CLP regulation (No 1272/2008 and its adaption 286/2011) also states that classification should be based on the ErC50. Thus only the effects based on growth rate are presented in the above "effects concentration" table. A summary of the EC50 and NOEC resuts based on all three effects (growth rate, yield and biomass is provided below:
Based on geometric mean measured test concentrations, the 72-hour EyC50 and the 0-72 hour EbC50 and ErC50 values were calculated to be 2.715, 2.926 and 6.289 mg/L, respectively.
The corresponding NOEC values for yield biomass and specific growth rate after 72 hours were 1.118 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a guideline study, conducted according to GLP, Super Muguet was found to have an NOEC (72 hr) of 1.118 mg/L. Based on geometric mean measured test concentrations, the 72-hour EyC50 and the 0-72 hour EbC50 and ErC50 values were calculated to be 2.715, 2.926 and 6.289 mg/L, respectively.
- Executive summary:
The effects of Super Muguet on the growth of the unicellular green alga, Psuedokirchneriella subcapitata, were determined during a 72-hour growth inhibition toxicity test conducted in accordance with OECD Chemicals Testing Guideline No. 201 Algae, Growth Inhibition Test.
Based on geometric mean measured test concentrations, the 72-hour EyC50 and the 0-72 hour EbC50 and ErC50 values were calculated to be 2.715, 2.926 and 6.289 mg/L, respectively.
The corresponding NOEC values for yield biosmass and specific growth rate adter 72 hours were 1.118 mg/L.
All validility criterea were met therefore the test was considered valid.
The preferred observational end point in this study is algal growth rate inhibition because it is not dependent on the test design (ECHA guidance Chapter R.7b v1.1) and classification shall be based on the ErC50 (EU CLP regulation No 1272/2008 and its adaption 286/2011). In terms of the NOEC and EC10 values, there is a preference for use of the EC10 value because it is derived from the dose response curve. In contrast the NOEC depends on the experimental design (e.g. the concentrations used in the test). Thus the 72-h EC50 and EC10 based on growth rate are used for classification purposes.
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