Reaction products of alkenes, C10-C14-(branched and linear), C12-rich, with hydrogen and carbon monoxide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Alcohols, C11-14-iso-, C13-rich

EC Number:

271-235-6

EC Name:

Alcohols, C11-14-iso-, C13-rich

Cas Number:

68526-86-3

Molecular formula:

Unspecified

IUPAC Name:

Alcohols, C11-14-iso-, C13-rich

Details on test material:

- Name of test material (as cited in study report): Tridecanol A
- Physical state: liquid/colourless-clear
- Analytical purity: 99.95%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: All test concentrations were prepared separately. The test substance was injected by a microtiter-syringe in the test water. All concentrations were treated in an ultrasonic bath for about 3 min. and subsequently stirred for about 20 hours at 20 ± 2 °C.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Institut National de Recherche Chimique Appliquee, France), cultured under standard laboratory conditions
- Age at study initiation: 2 - 24 hours

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.2 - 3.2 mmol/L

Test temperature:

20 ± 2 °C

pH:

7.5 - 8.5

Dissolved oxygen:

- M4 medium is aerated for about 24 hours until saturation with oxygen is reached

Nominal and measured concentrations:

Nominal test concentrations: control, 0.156, 0.313, 0.625, 1.25, 2.5, 5.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, total volume, fill volume: glass test tubes with flat bottom, 20 ml, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Alkalinity: 0.8 - 1.0 mmol/L (up to pH 4.3)
- Ca/mg ratio: about 4:1
- Conductivity: 550 - 650 µS/cm²
- Culture medium different from test medium: No, synthetic culture medium M4 based on ultra pure, deionised water
- Intervals of water quality measurement:
- Temperature: continuously during the whole test period in a separate vessel close
- After 0 h in an additional replicate without daphnids and after 48 h in replicate 1 of each tested concentration the test vessels

OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h darkness
- Light intensity: artificial light with an intensity of about 1 - 2 µE/(m*m*s)

EFFECT PARAMETERS MEASURED: mobility recorded after 0, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.105 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.275 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 0.174 - 0,342 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

1.13 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Results on the basis of nominal concentrations:
EC0 (48h) = 0.156 mg/L
EC50 (48h) = 0.263 mg/L (CL 95%: 0.0324 - 0.585 mg/L)
EC100 (48h) = 1.25 mg/L