D-Glucitol, 1-deoxy-1-(dimethylamino)-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Remarks:

The guidelines of chemical testing good laboratory practices (HJ/T 155)”,

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

other: Gobiocypris rarus

Details on test organisms:

The Rare minnow, Gobiocypris rarus was used in the test on the basis of such important practical criteria as, for example, their ready availability throughout the year, ease of maintenance, convenience for testing and any relevant economic, biological or ecological factors. The test species of Rare minnow, Gobiocypris rarus (Batch No.: F20160215G), were obtained from a commercial fish supplier of Institute of Hydrobiology, Chinese Academy of Sciences.
Feeding fry were held at least for 12 days in holding tanks supplied with a continuous flow of aerated water before being used for testing. Fish to be used in the test were held for 7 days in water of the quality and temperature to be used in the test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

174 - 181 mg/L CACO3

Test temperature:

22.8 - 23.3 °C

pH:

7.62 - 7.76

Dissolved oxygen:

76 - 95 %

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

The results showed that under valid static test conditions, the 96 h-LC50 of Genamin DMG 75 to fish (Rare minnow, Gobiocypris rarus) is greater than the nominal concentration of 100 mg/L (measured water samples concentration was 102 mg/L), while the maximum concentration causing no mortality (96 h-LC0) was equal to the nominal concentration of 100 mg/L (measured water samples concentration was 102 mg/L). i.e.:
96 h-LC50 > 100 mg /L (measured water samples concentration was 102 mg/L);
96 h-LC0 = 100 mg/L (measured water samples concentration was 102 mg/L).

Executive summary:

Under static conditions,the acute toxicity of test substance(Genamin DMG 75)toRare minnow (Gobiocypris rarus)was conductedaccording to:“The guidelines for the testing of chemicals” (HJ/T 153-2004) and“The Guidelines for the Testing of Chemicals-Effects on Biotic Systems” (the 2nd edition.:Environment Press. 2013);with reference toProcedure 203 of the“Guidelines for Testing of Chemicals”of the OECD:“Fish, Acute Toxicity Test” (1992).

A range-finding test and then a limit test were performed respectively. Nominal concentrations of 1.00, 10.0 and 100mg/Lwere used in the range-finding test, while the nominal concentration of100 mg/Lwas used in the limit Test. Water samples taken from the control and the treatments in the limit test (0h, 24h, 48h, 72h & 96h) were analysed.Themean measured concentration of samples was 102mg/L.The analytical results showed that the concentration of the test substance was consistent in the test medium throughout the 96-hour test period(deviation within 20%). Thus a static procedure was reasonable.

In the range-finding test, 5 fish per treatment and no replicates were used. And 7 fishper treatmentwith 3 replicates were used in the limit Test.The testfishwereexposed for 96 hours to the testsolution.

During thelimittestperiod,the pHvaluesof the control mediumsand test mediums werebetween 7.62 and 7.76, the Dissolved Oxygen (DO) values varied from 76 % and 97 % of theair saturationat the test temperature, and the total hardness was in the range of 174 mg (CaCO₃)/L to 181 mg (CaCO₃)/L. During the test, the temperature of the test mediums were maintained in the range of 22.8 ºC to 23.2 ºC, and all fishes in the control group were normal. With thesameconditions, potassium dichromate (K₂Cr₂O₇)wasused as thepositive controlsubstance, and the resulting 24 h-LC₅₀was327mg/L.So the study met the acceptability criteria prescribed by the protocol and Test Guidelines (The mortality of control ≤ 10%;pH:6.0~8.5; dissolved oxygenconcentration:>60% of the air saturation value;total hardness:10~250 mg(CaCO₃)/L; temperature: (23±2) ºC;24 h-LC₅₀ofK₂Cr₂O₇inthe range of 200 to 400 mg/L). Therefore the test was considered valid.

During the test period, all fishin the control groups and treated groupswere alive and appeared normal.The results showed that undervalidstatic test conditions, the 96 h-LC₅₀ofGenamin DMG 75to fish (Rare minnow,Gobiocypris rarus)was greater than the nominal concentration of 100mg/L(measured water samples concentration was 102 mg/L), while the maximum concentration causing no mortality (96 h-LC₀) was equal to the nominal concentration of 100mg/L(measured water samples concentration was 102 mg/L). i.e.:

96 h-LC₅₀>100 mg/L(measured water samples concentration was 102 mg/L);

96 h-LC₀=100 mg/L(measured water samples concentration was 102 mg/L).

Description of key information

The results showed that under valid static test conditions, the 96 h-LC50 of Genamin DMG 75 to fish (Rare minnow, Gobiocypris rarus) is greater than the nominal concentration of 100 mg/L (measured water samples concentration was 102 mg/L), while the maximum concentration causing no mortality (96 h-LC0) was equal to the nominal concentration of 100 mg/L (measured water samples concentration was 102 mg/L). i.e.:

96 h-LC50 > 100 mg /L (measured water samples concentration was 102 mg/L);

96 h-LC0 = 100 mg/L (measured water samples concentration was 102 mg/L).