[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 4-7, 2010

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Fatty acids C10-20, reaction product with diethylenetriamine

EC Number:

940-308-0

IUPAC Name:

Fatty acids C10-20, reaction product with diethylenetriamine

Test material form:

liquid: viscous

Details on test material:

Test substance: Fatty acids, C10-20 neo, reaction product with diethylenetriamine
Old name if the test substance: MK195K
Stability: No particular stability concerns
Solubility: Insoluble
Odor: Aromatic
Storage: room temperature

Specific details on test material used for the study:

Old names of the test substance: Naphthenic acids, reaction products with diethylenetriamine; MK195K.

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95%CL (2.3-3.7mg/L)

Any other information on results incl. tables

Fatty acids, C10 -20 neo, reaction product with diethylenetriamine was insoluble in seawater; upon addition to seawater in the mixing jars the majority of the material would float or be caught up in the stirring vortex. During the 48 hour course of the study, normal behavior was observed in the control(s). A twenty four hours of exposure, mortality of one test organism was observed at 1.25mg/L. Complete immmobilization (100%) was observed in the top concentration of 10mg/L, complete mortality was observed in all replicates. The 48 hour EC50 and 95% confidence limits for Fatty acids, C10 -20 neo, reaction product with diethylenetriamine to Daphnia magna were determined to be 2.9mg/L.

Applicant's summary and conclusion

Conclusions:

The scope of the study is to evaluate the acute toxicity of water accomodated fractions of Fatty acids, C10 -20 neo, reaction product with diethylenetriamine to
freshwater invertebrates, Daphnia Magna, under static conditions.
Chemical analysis of the water soluble fractions was not performed. All values were based upon nominal concentrations of the substance in test water.
The test substance is an UVCB substance, insoluble in water; upon addition to seawater in the mixing jars the majority of the material would float (relative density: 0.83-1.01) or be caught up in the stirring vortex. Furthermore it has surfactant properties, tends to accumulate at the interfaces and to form aggregates, such as micelles, in the bulk aqueous phase.
These characteristics do not allow to to conduct tests appropriately to obtain exposure of the test species.
The FND ( Fatty Nitrogen Derived) Amides chemicals should be considered to be toxic to aquatic species under conditions that allow contact of the chemicals with the organisms. This toxicity is common not only to the FND Amides chemicals but also to the FND Amines, Cationics, and Nitriles Categories. Further, the high toxicity is consistent with that of a large body of surfactants (e.g. alcohol ethoxylates, alkylphenol ethoxylates, sodium lauryl sulfate, and others).