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EC number: 700-208-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-07-08 - 2008-11-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to an internationally accepted guideline. All study parameters are based on the specific guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- Preparation and incubation of the test solutions:
Solutions of the test item were freshly prepared at the start of the test using a stock solution. Preparation of the test item solutions:
The weighed amount of the test item was mixed with tap water to prepare seven test item solutions immediately before application.
Preparation of the control solution:
24 ml. of synthetic sewage feed (prepared in accordance with OECD guideline 209), 426 mL tap water and 300 mL sludge suspension were pipetted into an incubation vessel (1 L glass flask).
Preparation of the test solutions:
100 mL of the respective test item solution, 24 mL of synthetic sewage feed (prepared in accordance with OECD guideline 209), 326 mL tap water and 300 mL sludge suspension were pipetted into an incubation vessel (1 L glass flask).
Afterwards, the total mixture (750 mL) per vessel was aerated with oil-free air with an air flow of 0.5 to 1 L per minute. The incubation of the test solution was performed at a temperature of 20 ± 2 °C over a time span of three hours (contact time). - Test organisms (species):
- activated sludge
- Details on inoculum:
- As microbial source (inoculum) for the test, activated sludge from the municipal sewage treatment plant 04451 Borsdorf, Germany was used (date of collection: 16.07.2008).
Before test start, the collected sludge was washed with tap water to remove possible disturbing substances (e.g. respiration inhibitors).
For this purpose the sludge was centrifuged and the developed supernatant was decanted. Afterwards, tap water was added and mixed with the sludge. This suspension was centrifuged again and the supernatant was decanted. The washing procedure was repeated three times.
After the washing procedure was finished, a small amount of the washed sludge (approx. 10 g) was weighed and dried for the determination of the sludge dry weight (sludge d.w.). From this result an activated sludge suspension was prepared with a concentration of about 4 g sludge dry weight per litre tap water.
Since the activated sludge could not be used for the test on the day of collection (16.07.2008), 50 ml_ synthetic sewage feed (prepared in accordance with OECD guideline 209) were added to each litre of the sludge suspension and then aerated at 20 ± 2 °C until the test was started (17.07.2008).
Before use, the pH-value of the activated sludge suspension was measured as 6.5 (required pH 6.0 - 8.0). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- 6.5
- Dissolved oxygen:
- Aeration of the test vessels: continuously during the contact time
- Nominal and measured concentrations:
- Nominal: 100, 375, 750, 1500, 3000, 6000 and 12000 mg test item/L
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Results with reference substance (positive control):
- To verify the sensitivity of the test system the reference item 3,5-dichlorophenol was tested in a separate study (BioChem project No,: R 08 10 48 001 D) on the same batch of activated sludge like the test item at concentrations of 2.5, 5.0, 10.0, 20.0 and 40.0 mg/L. The EC50 was calculated to be 6.4 mg/L (required 5 - 30 mg/L).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity of N-[3(5)-Methyl-1 H-pyrazol-1 -yl-methyl]acetamide to microorganism was determined according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test). The EC50of the test item after 3 hours of exposure to the micro-organisms of activated sludge was calculated to be 2120 mg/L.
- Executive summary:
The toxicity of N-[3(5)-Methyl-1 H-pyrazol-1 -yl-methyl]acetamide to microorganism was determined according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test). The EC50of the test item after 3 hours of exposure to the micro-organisms of activated sludge was calculated to be 2120 mg/L.
Reference
Effects on activated sludge after treatment with P70/05/S-4
Treatment group |
O2 consumption [mg/L/10 min] |
O2 consumption [mg/L 60 min] per replicate |
mean |
Inhibition [%] |
Control |
4.09 4.25 |
24.54 25.50 |
25.02 |
|
100 mg P70/05/S-4/L |
2.91 |
17.46 |
- |
30.2 |
375 mg P70/05/S-4/L |
2.74 |
16.44 |
- |
34.3 |
750 mg P70/05/S-4/L |
2.66 |
15.96 |
- |
36.3 |
1500 mg P70/05/S-4/L |
2.20 |
13.20 |
- |
47.2 |
3000 mg P70/05/S-4/L |
1.97 |
11.82 |
- |
52.8 |
6000 mg P70/05/S-4/L |
1.67 |
10.02 |
- |
60.0 |
12000 mg P70/05/S-4/L |
1.42 |
8.52 |
- |
65.9 |
Description of key information
The toxicity of N-[3(5)-Methyl-1 H-pyrazol-1 -yl-methyl]acetamide to microorganism was determined according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test). The EC50 of the test item after 3 hours of exposure to the micro-organisms of activated sludge was calculated to be 2120 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2 120 mg/L
Additional information
The EC50 of the test item after 3 hours of exposure to the micro-organisms of activated sludge was calculated to be 2120 mg/L.
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