1-(4-Methoxyphenyl)ethanamine

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

other information

Study period:

2007-11-29 to 2007-11-30 (experimental phase)

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

82796-69-8

Cas Number:

82796-69-8

IUPAC Name:

82796-69-8

Details on test material:

- Batch-Identification: 76584075L0
- Purity: 99.8 area-%
- Homogeneity: Homogeneous
- Storage stability: Expiry date: 17 January 2009
- Physical state/ Appearance: Liquid / colorless, clear

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 2 g of the test substance were added to about 800 mL of demineralized water and stirred at room temperature until the test substance was completely dissolved. The stock solution was made up to 1 L with demineralised water. The stock solution appeared colorless and clear.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Source and preparation of the inoculum: Activated sludge from the municipal wastewater treatment plant of the city Mannheim/Baden Württemberg (Germany). It was collected at 29 Nov 2007 from the aeration tank of the plant. The activated sludge suspension was washed thrice with tap water, sieved with a fine woven mesh (mesh size about 1 mm). This suspension was adjusted to a concentration of 7.5 g/L dry weight and pre-aerated over night. After the pre-aeration the lost water was replaced. This suspension was added to the test vessels to obtain a sludge concentration of 1.5 g/L dry substance.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

180 min

Test conditions

Test temperature:

20 ± 2 °C

pH:

Adjusted to 7.2

Dissolved oxygen:

Oxygen concentration during aeration: > 2.5 mg/L
Oxygen concentration immediately before measurement: > 6.5 mg/L

Nominal and measured concentrations:

Nominal concentrations: 62.4, 125, 250, 500 and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250mL)
- Fill volume: 250 mL
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of medium: According to guideline
- Intervals of water quality measurement: At test start and at test end

EFFECT PARAMETERS MEASURED: Respiration inhibition

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

yes

Remarks:

3,5 dichlorophenole

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

180 min

Dose descriptor:

EC10

Effect conc.:

120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

230 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

740 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 4

Duration:

180 min

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes