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Diss Factsheets
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EC number: 700-937-1 | CAS number: 1312021-45-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data based on the inhibition control of a ready biodegradability study. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2012).
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Source of inoculum/activated sludge:
The sludge was taken from a biologic sewage treatment plant which is treating mostly domestic sewage. Sludge was taken on Sep 7th, 2012, from the activation basin of ESN (Stadtentsorgung Neustadt) sewage treatment plant, located in Altenschemel, NW-Lachen - Speyerdorf in Germany.
- Pretreatment: The sludge was filtrated, washed with tap water twice, then washed with resuspended test medium. Then aerated for ≥ 12 hours.
- Concentration of sludge: 25,0 mg dry matter/L, determined by 4120 mg suspended sludge/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Details on test conditions:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 20.5 - 23.0 °C
- pH: 6.94 - 7.62
- pH adjusted: no
- Inoculum concentration: 25 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 2000 mL glass flasks
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: The test vessels were aerated with purified (by activated charcoal), CO2-scrubbed, moistened air. The scrubbing of carbon dioxide was achieved by bubbling the purified air through a flask containing 1.5 m-NaOH. To control the absence of CO2, the air was then led through a flask containing a solution of Ba(OH)2 before reaching the test vessels.
- Details of trap for CO2 and volatile organics if used: flask containing a solution of Ba(OH)2
SAMPLING
- Sampling frequency: Sampling was done on days 0, 2, 4, 7, 9, 11, 14, 18, 22 and 29
- Sampling method: 10 samples of 1 mL each were taken from each front scrubber flask. On day 29, samples from both flasks were taken.
- Reference substance (positive control):
- yes
- Remarks:
- Aniline
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 34.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Result of toxicity control from ready biodegradability test
- Results with reference substance (positive control):
- Aniline was chosen as positive control.
Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L). The test was left running for 28 days.
All validity criteria were met. Degradation of the positive control was 63 % after fourteen days.
Reference
The toxicity control attained 73.7% degradation after 14 days of incubation. Thus, the test item is not toxic to the inoculum. An inhibtion of of the activated sludge microorganisms is not expected.
The test item concentration in the toxicity control of 34.9 mg/L can be used as NOEC value for the toxicity to aquatic microorganisms.
Description of key information
NOEC (14 d): 34.9 mg/L (nominal, OECD 301B)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 34.9 mg/L
Additional information
Studies investigating the toxicity to aquatic microorganisms are not available. The assessment is therefore based on the data reported in the available ready biodegradability tests on the substance. A substance can be assumed to be not inhibitory to aquatic microorganisms, if in the toxicity control of a ready biodegradation test, more than 25% degradation based on O2 consumption or CO2 evolution occurred within 14 days (OECD guideline 301). This was clearly the case in all three available ready biodegradability test. Thus, the inhibition control of the available screening test following OECD 301 B was used to derive a NOEC for the toxicity to aquatic microorganisms.
The inhibition control included the available ready biodegradability test according to OECD 301B attained 73.7% degradation after 14 days. The test item concentration in the toxicity control was 34.9 mg/L, the concentration of the reference substance Aniline was 25.2 mg/L. The test item concentration of 34.9 mg/L can be used as NOEC.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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