Benzenemethanamine, .alpha.,4-dimethyl-, (.alpha.R)-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 June 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

D-α-methylbenzylamine

EC Number:

223-423-4

EC Name:

D-α-methylbenzylamine

Cas Number:

3886-69-9

Molecular formula:

C8H11N

IUPAC Name:

1-phenylethanamine

Details on test material:

- Name of test material (as cited in study report): (R)-(+)-1-Phenylethylamin
- Substance type: active substance
- Physical state: liquid
- Analytical purity: 99.6 area-% (analytical report: ZAX 98L00289)
- Lot/batch No.: 68-0655 (date of production: 09 Feb. 1997)
- Stability under test conditions: instable against acids and alkali
- Storage condition of test material: room temperature
- Other:

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The substance was added directly to the test vessels.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Laboratory culture: yes, municipal activated sludge taken from a laboratory wastewater treatment plant fed with municipal sewage.
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated
for 24 hours. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Test conditions

Test temperature:

20±2 °C

Dissolved oxygen:

Oxygen concentration during aeration: > 2.5 mg/L
Oxygen concentration immediately before measurement of oxygen consumption: > 6.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel
- Material, size, headspace, fill volume: glass, nominal volume: 250 mL, fill volume 250 mL
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium

OTHER TEST CONDITIONS
- Adjustment of pH: yes

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

ca. 550 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

- Results with reference substance valid?: yes
- Relevant effect levels: 30-min EC20: ca. 1.8 mg/L; 30-min EC50: ca. 6.7 mg/L; 30-min EC80: ca. 60 mg/L

Any other information on results incl. tables

Respiration data:

Treatment	BC1	BC2	BC3	BC (mean value)	TS1	TS2	TS3	RS1	RS2	RS3
Concentration of test substance (mg/L)	-	-	-	-	1000	500	250	1	10	100
O2 consumption rate (mg O2/L*h)	30	28	28	29	18	24	26	27	12	4
Specific O2 consumption rate (mg O2/g*h)	30	28	28	29	18	24	26	27	12	4
Change in oxygen consumption rate compared to blank control (%)	-	-	-	-	-38	-17	-10	-7	-59	-86
pH value*	6.4	6.5	6.6		9.8	9.6	8.9	6.5	6.6	6.5

* The pH value has been measured and adjusted to pH 7.0 to 8.0 before the addition of activated sludge.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes