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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
10-13 August 2009
Reliability:
1 (reliable without restriction)

Data source

Reference
Title:
Unnamed
Year:
2009
Report date:
2009

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-[(2,4-dichloro-5-methoxyphenyl)amino]-6-methoxy-7-[3-(4-methylpiperazin-1-yl)propoxy]quinoline-3-carbonitrile
EC Number:
700-455-1
Cas Number:
380843-75-4
Molecular formula:
C26H29Cl2N5O3
IUPAC Name:
4-[(2,4-dichloro-5-methoxyphenyl)amino]-6-methoxy-7-[3-(4-methylpiperazin-1-yl)propoxy]quinoline-3-carbonitrile

Sampling and analysis

Details on sampling:
During the project singular samples for possible analysis were taken from all test concentrations and the control according to the schedule below. In addition, the filter containing the undissolved material was kept for possible analysis. The method of analysis is described in the appended Analytical Report (Appendix IV).
Frequency at t=0 h, t=24 h and t=72 h
Volume 9 ml
Storage Samples were stored in a freezer until analysis.
At the end of the exposure period, the replicates with algae were pooled at each concentration before sampling. Compliance with the Quality criteria regarding maintenance of actual concentrations was demonstrated by running a test vessel at the highest substance concentration but without algae and samples for analysis were taken at the start, after 24 hours of exposure and at the end of the test period.
Additionally, singular reserve samples of 9 ml were taken from all test solutions for possible analysis. If not already used, these samples were stored in a freezer for a maximum of three months after delivery of the draft report, pending on the decision of the sponsor for additional analysis.

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
strain: NIVA CHL 1

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
72 h

Test conditions

Test temperature:
Continuously in a temperature control vessel.
pH:
At the beginning and at the end of the test in the control and the highest concentration. The pH of the solutions should preferably not deviate by more than 1.5 units during the test.
Reference substance (positive control):
yes

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.15 mg/L
Basis for effect:
growth rate

Applicant's summary and conclusion

Conclusions:
Under the conditions of the present study with Pseudokirchneriella subcapitata, no reduction of growth rate was recorded at any of the concentrations of WAY-173606 (crystallized) tested. The yield was significantly inhibited at a loading rate of 10 mg/l.
The EC50 for both growth rate reduction (ERC50: 0-72h) and the EC50 for yield inhibition (EYC50: 0-72h) were beyond the range tested, i.e. exceeded a TWA concentration of 0.067 mg/l.
Due to the very low solubility of WAY-173606 (crystallized) in water, concentration levels that might be toxic for algae could not be reached.