Reaction mass of 1-chlorobut-2-ene and 3-chlorobut-1-ene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-study according to guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of (E)-1-chlorobut-2-ene and 3-chlorobut-1-ene

EC Number:

908-820-9

Molecular formula:

not applicable

IUPAC Name:

Reaction mass of (E)-1-chlorobut-2-ene and 3-chlorobut-1-ene

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
- Water solubility (under test conditions): not applicable, hydrolysis
- Stability in water: hydrolysis




- Results of test for ready biodegradability: not ready biodegradable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

For stability control a series of the concentrations tested, was made up in the same way as the concentrations for biological part, but in separate vessels without test organisms ans NaHCO3
Sampling time : 0 and 48h

Test solutions

Vehicle:

no

Details on test solutions:

For stock solution, 1.1 ml of the test item were mixed with 1000 mL synthetic fresh water without NaHCO3 for about 1h in an open vessel under a laboratory hood and than the vessel was closed. The resulting solution served as stock solution with a measured analytical value of 420 mg TOC/L resp. 793 mg test item/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Strauss clone 5
- Source: inhosue breeding (origined from Bayer AG, 1991)
- Age at study initiation (mean and range, SD): 24h
- Method of breeding: females maintained in M4 medium in 1L beakers, at two to three day intervals, teh excuviae are sphoned off and the water is changed, offspring is removed from the breeding vessels; in a four week interval, offspring is isolated from the vessel and serve as a basis for further breeding
- Feeding during test
no


ACCLIMATION
- Acclimation period: 24h in synthetic water

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

no

Test conditions

Hardness:

14°dH

Test temperature:

20 +- 1°C

pH:

begin of the test: control: 8.1, concentration dependent decrease of a pH with 8.1 (2.77 mg/L) down to 3.9 at 79.3 mg/L
end of the test: control: 7.8, concentration dependent decrease with 7.4 (2.77 mg/L) down to 4.0 (79.3 mg/L), no major difference for the two endpoints measured

Dissolved oxygen:

Begin of the test: 7.7-8.1
End of the test: 7.8-8.7

Salinity:

no

Nominal and measured concentrations:

measured test concentrationss (DOC) after 48h ranges from 87-101% compared to the initial DOC values, therefore, the bological values were calculated with the nominal concentrations

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: closed (gas tight septum)
- Material, size, headspace, fill volume: 22 mL head-space test tubes volume inside the test vessel was increased to 20 mL resulting in a reduced gas phase
- Aeration: no
- No. of organisms per vessel: 5 organisms
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: not reported
- Ca/mg ratio: 4:1
- Na/K ratio: 10:1

- Culture medium different from test medium: no



OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: dark
- Light intensity: not applicable


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :


TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study: no details reported in the final report

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

61 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: not given

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

42 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: not given

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

28 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
- The high toxicity at the highest concentration may be due to the low pH level (3.9-4.0)

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Mortality: yes
- EC50/LC50: 1 mg/L < 50% effect, 2 mg/L >50% effect
- Other: Reference item was tested quartely

Reported statistics and error estimates:

Due to the strong dose-response relationship, the EC50 values after 24h and 48h were calculated graphically.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under conditions tested , the LC50 value after 48h of test item and its hydrolysis products is 42 mg/L. The toxicity observed can be regarded as pH effects (pH < 6.2 at concentrations >= 47.6)