Reaction mass of iodine and 2-Pyrrolidinone, 1-ethenyl-, homopolymer

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reference substance 001

EC Number:

607-771-8

Cas Number:

25655-41-8

Molecular formula:

C6H9I2NO

Details on test material:

- Name of test material (as cited in study report): PVP-lod 30/06
- Lot/batch No.: Lot: G2295BPZ0
- Analytical purity: 97.2 g/100 g

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by stirring the test substance in test medium for about 10 min at approximately 20 ± 2°C . The nominal concentration of the stock solution was 11,3 rng/I . The test solutions were prepared by diluting the stock solution.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Source (laboratory, culture collection): CHODAT SAG 86.81

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

23 ± 2 °C

pH:

7.8 - 8.0

Nominal and measured concentrations:

Nominal test substance concentrations:
control, 0.313, 0.625, 1.25, 2.5, 5, 10 mg/l

Mean measured concentrations:
0.35, 0.781, 1.60, 3.45, 6.70, 12.5 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flasks (250 ml) plugged with gas permeable siliconsponge caps
- Fill volume: 100 ml
- Initial cells density: 10000
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: test medium was prepared according to OECD Guideline for Testing of Chemicals, No. 201 Algal growth inhibition test


OTHER TEST CONDITIONS
- Photoperiod: continous illumination
- Light intensity and quality: artificial light, type universal white (OSRAM L 25), about 60 - 120 µE/(m²s) at a wave length of 400 - 700 nm
- Salinity (for marine algae):

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber (Neubauer improved)
- Chlorophyll measurement: in vivo chlorophyll-a-fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

4.91 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

3.62 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate