Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 604-636-5 | CAS number: 148477-71-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-04-20 to 2004-05-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on regulatory testing procedures for pesticides with nontarget arthropods (Barrett et al. 1994).
- Version / remarks:
- 1994
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: SECOFASE- Final Report, improvement and standardisation of test systems forassessing sub-lethal effects of chemicals on fauna in the soil ecosystem (Lokke & van Gestel 1996)
- Version / remarks:
- 1996
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Details on preparation and application of test substrate:
- - Preparation of the test item stock solution. The test item was dissolved in a sufficient amount of deionised water to prepare a stock solution. This stock solution was diluted with deionised water to obtain one test item solution (= dosage solution) for each test item concentration. An appropriate amount of these aqueous test item solutions were mixed in the LUFA 2.1 soil. This amount of water/test item-mixture served also to adjust the final soil moisture of 40 - 60% of WHCmax.
- Mixing of the LUFA 2.1 soil until the test item solutions were homogeneously distributed. For each concentration an appropriate amount of soil was separated and 5.1 - 5.3 g fw (fresh weight) was filled into each test unit. The height of the soil column in the test units was approx. 3 mm. After the soil substrate was inserted, it was slightly compressed to equalise the surface. - Test organisms (species):
- Hypoaspis aculeifer
- Animal group:
- Acari (soil-dwelling predatory mite)
- Details on test organisms:
- The culture of Hypoaspis aculeifer CANESTRINI (Acari: Laelapidae) used in this test is kept at
ECT Oekotoxikologie GmbH since February 2002. The organisms were originally delivered by
MITOX Laboratories (Amsterdam, The Netherlands).
The mites were in the protonymphal stage (with an maximum age of 2 days); i.e. they were
taken from a synchronised culture. - Study type:
- laboratory study
- Total exposure duration:
- 14 d
- Test temperature:
- 25 ± 2°C
- pH (if soil or dung study):
- 4.7
- Details on test conditions:
- Mortality (exposure) period: The mites were exposed to the test item in LUFA 2.1, a standard soil obtained from Landwirtschaftliche Untersuchungs-und Forschungsanstalt Speyer, Germany.
Mating and reproduction period: Following the mortality period mites were tested on an uncontaminated layer of plaster of Paris (approximately 1.5 cm) darkened with activated carbon.
During mortality period the soil moisture was adjusted to 40 - 60% of the WHC max. The plaster of Paris was moistened with deionised water until the plaster was almost saturated. The units were passively ventilated.
During the mortality and mating period the water content of the LUFA 2.1 soil and the plaster of Paris was checked twice per week by reweighing the test units. Losses of water were compensated.
For all test periods the following conditions were observed:
Temperature: 25 ± 2 °C
Light cycle: permanent dark
The mites were fed with prey mites (e.g.Tyrophagus putrescentiae) or enchytraeids (e.g.Enchytraeus vavoulidou). Food was checked twice per week and was added on demand. - Nominal and measured concentrations:
- Control: 10.0, 31.6, 100.0, 316.0 and 1000.0 mg test item/kg soil (dw)
- Reference substance (positive control):
- yes
- Remarks:
- Reference item 5 mg dimeothate/kg soil (dw)
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Eight percent of adult mites died in the control. At all concentrations of the test item tested 6.3-15.0% mortality respectively was observed (corresponding to a corrected mortality according to Abbott (1925) between1.9 and 7.6%). Since the mortality observed with the test item was not higher than 15.0% the LR50 value could not be calculated.
At all tested concentrations of the test item, the highest mortality rate occurred at a concentration of 100.0mg test item/kg soil (dw) and was 7.6% higher than at the control but remained below the maximum recommended control mortality of 25.0% which is set as validity criterion. Thus no statistical analysis was performed and the NOEC Mortality was regarded to be >1000.0 mg test item/kg soil (dw). - Results with reference substance (positive control):
- After 14 days of exposure, 83.7% Abbott's (1925) corrected mortality of the adult mites was observed with the reference item group which was within the recommended range (50 -99.5%). Therefore, the observed effects of the reference item assures a high sensitivity of the test system.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LR50value could not be calculated.
The NOEC Mortality was >= 1000.0 mg test item/kg soil (dw).
The NOEC Reproduction was >= 1000.0 mg test item/kg soil (dw). - Executive summary:
Eight percent of adult mites died in the control. At all concentrations of the test item tested 6.3-15.0% mortality respectively was observed (corresponding to a corrected mortality according to Abbott (1925) from -1.9 to 7.6%).
Since the mortality observed with the test item was not higher than 15.0% the LR50 value could not be calculated.
At all tested concentrations of the test item, the highest mortality rate occured at a concentration of 100.0 mg test item/kg soil (dw) and was 7.6% higher than at the control but remained below the maximum recommended control mortality of 25.0% which is set as validity criterion.
Therefore, the NOEC Mortaility was regarded to be ≥ 1000.0 mg test item/kg soil (dw). Reproduction was examined only for the two highest concentrations of the test item which caused less than 50% mortality (i.e. 316.0 and 1000.0 mg test item/kg soil (dw)). Statisticalanalysis (Welch-t test for inhomogeneous variances; 1-sided smaller, p ≤ 0.05) showed no significant difference concerning the cumulative number of juveniles per female after 7 days between the control and this two concentrations of the test item tested.
Therefore, the NOEC reproduction was regarded to be ≥ 1000.0 mg test item/kg soil (dw)
Reference
Table one
Mortality of the adult mites after 14 days
Concentration [mg/kg soil (dw)] | Average Mortality (%) | Corrected mortality (%) | Mean cummulative number of juveniles / females after 7 days [mean ± sd] | Reduction of Juveniles (%) |
Control | 8.0 | 0.0 | 18.1 ± 4.8 | 0.0 |
Reference | 85.0 | 83.7 | - - | - |
10.0 | 6.3 | -1.9 | - - | - |
31.6 | 6.3 | -1.9 | - - | - |
100.0 | 15.0 | 7.6 | - - | - |
316.0 | 13.8 | 6.3 | 17.3 ± 4.4 | 4.4 |
1000.0 | 8.8 | 0.8 | 17.4 ± 2.9 | 3.9 |
Adult Mortality | Reproduction | |||
LR50 [mg test item/ kg soil (dw)] | --- | --- | ||
LOEC [mg test item / kg soil (dw)] | --- | --- | ||
NOEC [mg test item/kg soil (dw)] | ≥ 1000.0 | ≥1000.0 |
Table two
Reproduction after a 7-day period
Concentration [mg test item/ kg soil (dw)] | Mean cummulative Number of Juveniles / Female after 7 days | Reduction of Juveniles [%] |
control | 18.1 ± 4.8 | 0.0 |
316.0 | 17.3 ± 4.4 | 4.4 |
1000.0 | 17.4 ± 2.9 | 3.9 |
Description of key information
In a 14-day tox study, Hypoaspis aculeifer, were exposed to concentrations of 10.0, 31.6,100.0, 316.0 and 1000.0 mg test item/kg soil dry weight (dw). As a formulation of Spirodiclofen was used in this study, the NOECMortality and NOECReproduction of >= 1000 mg/kg soil (dw) had to be coverted to >= 244 mg/kg soil (dw) to reflect the content of Spirodiclofen in the test item.
Key value for chemical safety assessment
- Long-term EC10, LC10 or NOEC for soil dwelling arthropods:
- 244 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.