Registration Dossier
Registration Dossier
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EC number: 603-894-6 | CAS number: 135108-88-2
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The toxicity of Formaldehyde, polymer with benzenamine, hydrogenated to activated sludge microorganisms was determined in a GLP study according to EC method C.11 (2008). On the basis of the effective concentrations describing the respiration inhibition of activated sludge caused by the test substance, Formaldehyde, polymer with benzenamine, hydrogenated was found to have an EC50 (3 h) of 187 mg/l (95% confidence interval: 137 – 251 mg/l).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 187 mg/L
Additional information
Test performance
Test was performed according to: Method C.11 - Activated Sludge Respiration Inhibition Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The preliminary test was performed using 5 concentrations in range 46 – 1 000 mg.L-1with a geometric factor of 2.2 to determinethe concentrations to be used in the main test.
In the main test, 5 concentrations of the test substance in geometric progression with factor of 2.0 (50, 100, 200, 400, 800 mg.L-1) were used together with two control experiments (without the test substance), one at the beginning and one at the end of the test.
3,5-dichlorophenol was used as reference substance. Five concentrations in geometric progression with a factor of 1.4 (5.2, 7.3, 10, 14 and 20 mg.L-1) were selected.
The abiotic decomposition was not detected even at the highest concentration of the test substance.
The pH value during the main test was in the range of 7.1 to 9.7.
Validity of test
The test met the following validity criteria:
- the respiration rates of two control experiments should not differ from each other by more than 15 %
- the EC50value of the reference substance should be in the range of 5 – 30 mg.L-1
The difference of two respiration
rates of control experiments at the beginning and the end
of the main test from each other was:
± 0.59 mg.L-1.h-1= ± 3.72 % (if the value of lower control 15.79 mg.L-1.h-1= 100 %)
The 3h-EC50value of reference substance, 3,5-dichlorophenol:
EC50= 8.1 mg.L-1(95% confidence interval: 7.6 – 8.6 mg.L-1)
Since all criteria for acceptability of the test were met, this study is considered to be valid.
Results of the respiration inhibition test
On the basis of the effective concentrations describing the respiration inhibition of activatedsludge caused by the test substance,Copolymer of aniline and formaldehyde, hydrogenated, the EC50was found to be:
EC50= 187 mg.L-1(95% confidence interval: 137 – 251 mg.L-1)
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