Ethanone, 1-(1-chlorocyclopropyl)-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

7 Oct - 11 Oct 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

TOC determination

Details on sampling:

- Concentrations: 0 and 100 mg/L
- Sampling schedule:
Control: on Oct. 7, Oct. 8, Oct. 9 and Oct. 10, 2002 in the freshly prepared medium; on Oct. 8, Oct. 9, Oct. 10 and Oct.l 1, 2002 after 24 h of exposure
Test concentration: on Oct. 7, Oct. 8, Oct. 9 and Oct. 10, 2002 in the freshly prepared medium; on Oct. 8, Oct. 9, Oct. 10 and Oct. 11, 2002 after 24 h of exposure

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To produce the only test concentration the test substance was added daily to 5 litres of dilution water, then treated with an ultra turrax for 60 sec. at 8000 rpm and afterwards stirred for 24 h on a magnetic stirrer.
- Controls: Yes
- Test concentration separation factor: Single concentration was used
- Evidence of undissolved material : Undissolved particles of the test substance were removed by filtration using a folded filter of pore size 7-12 um.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish (Danio rerio)
- Source: Aqua KloeGer
- Length at study initiation (n = 20): 3.21cm(S.D. = 0.25 cm)

ACCLIMATION
- Acclimation period: Stock held since Sept. 2, 2002 and acclimatised to the test conditions since then.
- Acclimation conditions: Same as test
- Type and amount of food during acclimation: Commercial fish food
- Feeding frequency during acclimation: Daily. Discontinued 24 h prior to test.
- Health during acclimation: < 2 % mortality
- Medication: None

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

11.4 °dH (=203.5mg/L CaCO3)

Test temperature:

21.0 - 22.6 °C

pH:

7.7 - 7.9

Dissolved oxygen:

96 - 99 %

Nominal and measured concentrations:

Nominal: 100 mg/L
Measured: 42 - 74 mg/L at 0 h and 38 - 48 mg/L at 24 h

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria holding 5 L of test media
- Aeration: gentle aeration via narrow glass tubes
- Renewal rate of test solution: daily
- No. of organisms per vessel: 10 animals per test concentration
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: 0.61 g body weight (wet weight) per litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water prepared according to the recommendations of ISO 7346
- Culture medium different from test medium: No. This freshly prepared standard dilution water is used for both, the maintenance of the test animals under flow-through conditions and the preparation of stock and test solutions of the test item.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light : 8 h dark

EFFECT PARAMETERS MEASURED: Mortalities are recorded at 24-hour intervals

TEST CONCENTRATIONS
- Spacing factor for test concentrations: Single concentration was used

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 49.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: based on geometric mean of all measured concentrations

Details on results:

- Other abnormalities: None
- Mortality of control: 0 %

Sublethal observations / clinical signs:

Chemical analysis for control

Chemical analysis date	0 h Oct. 7	24 h Oct. 8	0 h Oct. 8	24 h Oct. 9	0 h Oct. 9	24 h Oct. 10	0 h Oct. 10	24 h Oct. 11
TOC values [mg/L]	<2	<2	<2	<2	<2	<2	<2	<2

 

Chemical analysis for 100 mg/L

Chemical analysis date	0 h Oct. 7	24 h Oct. 8	0 h Oct. 8	24 h Oct. 9	0 h Oct. 9	24 h Oct. 10	0 h Oct. 10	24 h Oct. 11
TOC values [mg/L]	31	22	21	19	37	20	29	24
test substance [mg/L]	62	44	42	38	74	40	58	48

Validity criteria for the measurement of the fish toxicity

Target condition according to guideline:	Actual condition according to the study:	Validity criteria met:
In the control(s) (dilution water control, solvent control), the mortality should not exceed 10% (or one fish, if fewer than 10 control fish are tested) at the end of the exposure	No mortality	Yes
The dissolved oxygen concentration should be ≥60% of the air saturation value in all test vessels throughout the exposure	dissolved oxygen concentration was ≥60%	Yes
There should be evidence that the concentration of the test substance has been satisfactorily maintained, and preferably it should be at least 80% of the nominal concentration throughout the exposure. If the deviation from the nominal concentration is >20%, results should be based on the measured concentration (geometric mean in static and semi-static tests, arithmetic mean in flow-through tests; time weighted average, where applicable).	Initial measured concentration at 0 h was < 80 % of nominal concentration and was highly variable at each measurment time point. However, the LC50 was based on the geometric mean of the measured concentration during the 0 - 96 h exposure period.	Yes
During the acclimatisation period, mortalities are recorded, and the following criteria are applied: - mortalities of greater than 10% of the population in seven days: reject the entire batch; - mortalities between 5 and 10% of the population: acclimatisation is continued for seven additional days; and if there is more than 5% mortality during the second seven day period, reject the entire batch; - mortalities of less than 5% of population in seven days: accept the batch.	< 2 % mortality was observed during acclimatization phase	Yes
The pH should not vary by more than 1 unit.	The pH was between 7.7 - 7.9	Yes

 

Validity criteria fulfilled:

yes

Remarks:

See more details in the table in the section "Any other information on results incl. tables"

Conclusions:

No toxic effects against fish at a concentration of 100 mg/L (nominal concentration); 96h LC50 > 49.5 mg/L (geometric mean of measured concentration)

Executive summary:

In a 96-h acute toxicity study, Danio rerio were exposed to the test substance at a limit test concentration of 100 mg/L (nominal concentration) and control under semi-static conditions with daily renewal of test solution. The study was conducted in accordance to the EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 1 'Acute toxicity for Fish'. No toxic effects against fish were observed at the limit measured concentration of 49.5 mg/L (geometric mean) and therefore the LC50 >49.5 mg/L. The results are expressed in terms of the geometric mean of the measured concentrations during the 0 - 96 h exposure period. Chemical analysis was performed using TOC method and concentrations ranged from 42 - 74% of nominal values in freshly prepared medium and from 38 - 48% of nominal values after 24 hours of exposure. 

Description of key information

In a 96-h acute toxicity study, Danio rerio were exposed to the test substance at a limit test concentration of 100 mg/L (nominal concentration) and control under semi-static conditions with daily renewal of test solution. The study was conducted in accordance to the EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 1 'Acute toxicity for Fish'. No toxic effects against fish were observed at the limit measured concentration of 49.5 mg/L (geometric mean) and therefore the LC50 >49.5 mg/L. The results are expressed in terms of the geometric mean of the measured concentrations during the 0 - 96 h exposure period. Chemical analysis was performed using TOC method and concentrations ranged from 42 - 74% of nominal values in freshly prepared medium and from 38 - 48% of nominal values after 24 hours of exposure. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 49.5 mg/L